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Småskala plasmamembranpreparat for analyse av Candida albicans Cdr1-mGFPHis
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Småskala plasmamembranpreparat for analyse av Candida albicans Cdr1-mGFPHis

Article DOI: 10.3791/62592-v • 09:44 min • June 13th, 2021
June 13th, 2021 •
*1, *1, 1, 1,2, 3, 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland
* These authors contributed equally

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Denne artikkelen presenterer en liten plasmamembranisolasjonsprotokoll for karakterisering av Candida albicans ABC (ATP-bindende kassett) protein Cdr1, overekspressert i Saccharomyces cerevisiae. En protease-cleavable C-terminal mGFPHis dobbel tag med en 16-resters linker mellom Cdr1 og taggen ble designet for å lette rensing og vaskemiddel-screening av Cdr1.

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