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放线菌素D处理SiHa宫颈癌细胞中凋亡生物标志物的流式细胞术分析
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Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
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放线菌素D处理SiHa宫颈癌细胞中凋亡生物标志物的流式细胞术分析

DOI:
10.3791/62663-v

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13:53 min

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August 26, 2021

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, ,
1Department of Chemical Pathology, Faculty of Health Sciences,University of Pretoria, 2Tshwane Academic Division,National Health Laboratory Service

Capitoli

  • 00:04Introduction
  • 00:43Preparation of Cell Culture and Treatments for Flow Cytometry
  • 02:56Enumerating Cells Using the Viability Assay
  • 03:36Staining Cells for Flow Cytometry
  • 08:00Run Samples on the Flow Cytometer
  • 10:04Post-Acquisition and Statistical Analysis
  • 11:20Results: Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
  • 13:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

细胞凋亡可以通过早期和晚期凋亡生物标志物的流式细胞术分析来表征。使用台式流式细胞仪分析放线菌素D处理后宫颈癌细胞系SiHa的凋亡生物标志物。

Tags

Flow CytometryApoptosisActinomycin DSiHa Cervical Cancer CellsBiomarkersAnnexin VCell CultureTrypsinPhosphatidylserineDNA Binding Dyes

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