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A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft

Article DOI: 10.3791/63114-v • 08:30 min • February 14th, 2022
February 14th, 2022 •
1, 2, 3, 1, 1, 1, 1, 4, 3, 2, 5, 6, 2, 5, 7, 1, 1, 1, 3
1Division of Cardiothoracic Surgery, Department of Surgery, Duke University, 2Perfusion Services, Duke University, 3Division of Surgical Sciences, Department of Surgery, Duke University, 4Division of Laboratory Animal Resources, Duke University, 5Gift of Hope Organ and Tissue Donor Network, 6College of Veterinary Medicine, North Carolina State University, 7TransMedics, Inc

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We present a protocol for utilizing a normothermic ex vivo sanguinous perfusion system for the delivery of therapeutics to an entire cardiac allograft in a porcine heterotopic heart transplant model.

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Porcine Heterotopic Heart Transplantation Therapeutic Delivery Cardiac Allograft Extended Periods Of Time Cardiac Allograft Rejection Primary Graft Dysfunction Postoperative Immunosuppression Lifetime Of A Cardiac Allograft Transplant Procedure Ex Vivo Perfusion Incision Electrocautery Pectoralis Major Sternotomy
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