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Bioengineering

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Light-Controlled Fermentations for Microbial Chemical and Protein Production

Article DOI: 10.3791/63269-v • 08:37 min • March 22nd, 2022
March 22nd, 2022 •
*1, *1, 1,2,3,4
1Department of Chemical and Biological Engineering, Princeton University, 2The Andlinger Center for Energy and the Environment, Princeton University, 3Department of Molecular Biology, Princeton University, 4High Meadows Environmental Institute, Princeton University
* These authors contributed equally

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Optogenetic control of microbial metabolism offers flexible dynamic control over fermentation processes. The protocol here shows how to set up blue light-regulated fermentations for chemical and protein production at different volumetric scales.

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Optogenetics Light-controlled Fermentations Microbial Chemical Production Protein Production Titers Inducers Light-based Control Optimization Three-phase Fermentations Multi-chromatic Control Saccharomyces Cerevisiae Strain OptoINVRT Plasmids Gene Deletion Strain Construction Linearize Plasmid PIGA One Promoter Lithium Acetate Transformation Methods HIS3 Auxotrophic Strain
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