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Journal / Biochemistry / Quantitative Microtubule Fractionation Technique…
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Biochemistry

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JoVE Journal Biochemistry
Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues
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Journal (Biochemistry)
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Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Article DOI: 10.3791/63358-v • 07:21 min • November 17th, 2023
November 17th, 2023 •
1,2, 1,2,3
1Department of Neuropathology, Faculty of Life and Medical Sciences, Doshisha University, 2Center for Research in Neurodegenerative Diseases, Doshisha University, 3Laboratory of Physiology and Anatomy, School of Pharmacy, Nihon University

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Microtubules, which are tubulin polymers, play a crucial role as a cytoskeleton component in eukaryotic cells and are known for their dynamic instability. This study developed a method for fractionating microtubules to separate them into stable microtubules, labile microtubules, and free tubulin to evaluate the stability of microtubules in various mouse tissues.

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Tubulin labile microtubule stable microtubule post-translational modification microtubule-associated protein fractionation microtubule-targeting agents
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