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Journal / Neuroscience / Tüm Beyin Tek Hücreli Görüntüleme ve MRG, Doku…
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Neuroscience

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JoVE Journal Neuroscience
Tüm Beyin Tek Hücreli Görüntüleme ve MRG, Doku Temizleme ve Işık Tabakası Mikroskobu Kullanarak Bozulmamış Yenidoğan Fare Beyinlerinin Analizi
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
Journal (Neuroscience)
Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Journal (Neuroscience)
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
 
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Tüm Beyin Tek Hücreli Görüntüleme ve MRG, Doku Temizleme ve Işık Tabakası Mikroskobu Kullanarak Bozulmamış Yenidoğan Fare Beyinlerinin Analizi

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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Bu protokol, iDISCO + kullanarak bozulmamış fare beyinlerinin manyetik rezonans görüntüleme, temizleme ve immünoetiketleme yöntemlerini açıklar, ardından ışık tabakası mikroskobu kullanılarak görüntülemenin ayrıntılı bir açıklaması ve NuMorph kullanılarak aşağı akış analizleri yapılır.

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