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Immunology and Infection

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Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1, 1, 3, 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center

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The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

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Intestinal Permeability Lucifer Yellow Apical-out Enteroid Model Quantitative Analysis Qualitative Analysis Paracellular Permeability Pathways 3D Model Necrotizing Enterocolitis Human Intestinal Disease Enteroid Model Androids Embedded In BMM AO Enteroid Generation Cell Recovery Solution (CRS) Microcentrifuge Tube
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