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Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay

Article DOI: 10.3791/64221-v • 07:39 min • July 26th, 2022
July 26th, 2022 •
1, 2, 2, 2, 3, 2, 1
1Department of Oral and Maxillofacial Surgery, University of Florida, Gainesville, 2Department of Orthodontics, University of Florida, Gainesville, 3Stoelting Co. Wood Dale

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This protocol describes the assessment of mechanical hypersensitivity in a rat model of neuropathic orofacial pain using an operant-based orofacial pain assessment device.

Tags

Nerve Injury-induced Mechanical Hypersensitivity Orofacial Operant Pain Assay OPAD Preclinical Pain Research Operant Reward Conflict Paradigm Nociception Learning Motivation Translational Validity Reflex-based Assays Automated Pain Assessment Device Animal Restraint Experimenter Bias CCI Surgery Infraorbital Nerve Milk Drip Trays Plexiglass Cages Metal Flooring Grates Wiring Bottle Holder Reward Milk Bottles Software Protocol Setup
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