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Bioengineering

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JoVE Journal Bioengineering
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Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids

Article DOI: 10.3791/64365-v • 09:23 min • March 10th, 2023
March 10th, 2023 •
1, *1, *1, 1, 1, 1, 1, 1, 2, *1, *1
1Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 2Amsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center
* These authors contributed equally

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Presented here is a set of protocols for the generation and cryopreservation of cardiac spheroids (CSs) from human-induced pluripotent stem cell-derived cardiomyocytes cultured in a high-throughput, multidimensional format. This three-dimensional model functions as a robust platform for disease modeling, high-throughput screenings, and maintains its functionality after cryopreservation.

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Generation High-throughput Screening Biobanking Human-induced Pluripotent Stem Cell-derived Cardiac Spheroids Workflow Maintenance Optical Analysis In Vitro Disease Models Cardiac Detachment Solution Cardiomyocytes Mechanical Dissociation Single Cell Suspension Replating Media Cassette Cell Counting
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