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In Vitro Method to Study Sex-Based Differences in Conjunctival Goblet Cells

Article DOI: 10.3791/64456-v • 06:28 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1
1Department of Ophthalmology Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School

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Phenol red-free/fetal bovine serum-free medium is a better option than advanced RPMI to eliminate exogenous hormones without altering the normal function of conjunctival goblet cells in the study of sex-based differences.

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In Vitro Method Study Sex-Based Differences Conjunctival Goblet Cells Dry Eye Disease Ocular Surface Health Prevalence Women Gel-Forming Mucin Secretion Exogenous Sex Hormones Consistent Results Physiological Function Postmortem Human Donors Culture RPMI Medium Fetal Bovine Serum (FBS) Phenol Red BSA Cellular Function Intracellular [Ca2+] Carbachol Stimulation
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