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Biochemistry

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JoVE Journal Biochemistry
Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures
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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Article DOI: 10.3791/65086-v • 06:48 min • August 18th, 2023
August 18th, 2023 •
1, 1, 2,3, 2, 3, 1
1Department of Biochemistry, Albert Einstein College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern Denmark, 3CelVivo ApS

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We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

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Biochemistry Clinostat Incubator 3D Cell Cultures Cancer Hepatocytes Non-cancer Hepatocytes Liver Cells Incubators For 3D Cells Cell Growth Monitoring Cell Growth Snapshots Spheroid Counting Spheroid Measurement THLE-3 Cell Line HepG2/C3A Cell Line Proteomics Experiments Cell Signaling Perturbation Respiratory Chain Metabolism Metal Detoxification Phenotypic Characterization Image Capture Proteomics Pipeline
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