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JoVE Journal Biology

Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Journal (Biology)

Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Journal (Biology)

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

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Decoding RNA Methylation's Role in Pancreatic Cancer - A Single-Base Resolution Study (Video) | JoVE

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

Article DOI: 10.3791/65352-v • 01:27 min • July 7th, 2023

July 7th, 2023 •

Szu-Ying Chen^1,2, Po-Hsien Huang^1,2

¹Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

Tags

Enriquecimiento de ARNm Preparación de la biblioteca de bisulfito-ARNm Secuenciación de próxima generación Modificaciones postranscripcionales de ARN Regulación del ARN Células eucariotas Modificaciones del ARN 5-metilcitosina Metiltransferasas de ARN Enfermedades Cánceres Secuenciación de bisulfito en todo el transcriptoma Niveles de metilación de citosina Purificación de ARN poli(A) Reacción de bisulfito Preparación de la biblioteca Sitios de modificación de 5-metilcitosina de ARNm Evaluación de la cantidad y calidad del ARN Monitoreo de la integridad del ARN Alta calidad Bibliotecas de secuenciación

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