Name: Marcatura in vivo di isotopi stabili per lidentificazione con spettrometria di massa dei metaboliti paterni trasferiti dallo spermatozoo allovocita durante la fecondazione (Video) | JoVE Journal
Uploaded: 2025-06-17T14:00:00.000+00:00
Duration: 5 min 55 s
Description: Scopri la marcatura in vivo con isotopi stabili per identificare i metaboliti paterni trasferiti durante la fecondazione. Questo metodo rivela il ruolo epigenetico dello spermatozoo nello sviluppo embrionale precoce.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Wang, Y., Liu, H., Sun, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lamarck+rises+from+his+grave%3A+Parental+environment-induced+epigenetic+inheritance+in+model+organisms+and+humans%5BTitle%5D)+AND+%22Biol+Rev%22%5BJournal%5D)">Lamarck rises from his grave: Parental environment-induced epigenetic inheritance in model organisms and humans.</a> Biol Rev. 92 (4), 2084-2111 (2017).</li>
<li>Champroux, A., Cocquet, J., Henry-Berger, J., Drevet, J. R., Kocer, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+decade+of+exploring+the+mammalian+sperm+epigenome%3A+Paternal+epigenetic+and+transgenerational+inheritance%5BTitle%5D)+AND+%22Front+Cell+Dev+Biol%22%5BJournal%5D)">A decade of exploring the mammalian sperm epigenome: Paternal epigenetic and transgenerational inheritance.</a> Front Cell Dev Biol. 6, 50(2018).</li>
<li>Horsthemke, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+critical+view+on+transgenerational+epigenetic+inheritance+in+humans%5BTitle%5D)+AND+%22Nat+Commun%22%5BJournal%5D)">A critical view on transgenerational epigenetic inheritance in humans.</a> Nat Commun. 9 (1), 2973(2018).</li>
<li>Schagdarsurengin, U., Steger, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epigenetics+in+male+reproduction%3A+Effect+of+paternal+diet+on+sperm+quality+and+offspring+health%5BTitle%5D)+AND+%22Nat+Rev+Urol%22%5BJournal%5D)">Epigenetics in male reproduction: Effect of paternal diet on sperm quality and offspring health.</a> Nat Rev Urol. 13 (10), 584-595 (2016).</li>
<li>Skinner, R. C., Gigliotti, J. C., Ku, K. -M., Tou, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+comprehensive+analysis+of+the+composition%2C+health+benefits%2C+and+safety+of+apple+pomace%5BTitle%5D)+AND+%22Nutr+Rev%22%5BJournal%5D)">A comprehensive analysis of the composition, health benefits, and safety of apple pomace.</a> Nutr Rev. 76 (12), 896-909 (2018).</li>
<li>King, S. E., Skinner, M. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epigenetic+transgenerational+inheritance+of+obesity+susceptibility%5BTitle%5D)+AND+%22Trends+Endocrinol+Metab%22%5BJournal%5D)">Epigenetic transgenerational inheritance of obesity susceptibility.</a> Trends Endocrinol Metab. 31 (7), 478-494 (2020).</li>
<li>Martínez Duncker Rebolledo, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sperm+DNA+methylation+defects+in+a+new+mouse+model+of+the+5%2C10-methylenetetrahydrofolate+reductase+677C%26gt%3BT+variant+and+correction+with+moderate+dose+folic+acid+supplementation%5BTitle%5D)+AND+%22Mol+Hum+Reprod%22%5BJournal%5D)">Sperm DNA methylation defects in a new mouse model of the 5,10-methylenetetrahydrofolate reductase 677C&gt;T variant and correction with moderate dose folic acid supplementation.</a> Mol Hum Reprod. 30 (4), gaae008(2024).</li>
<li>Lismer, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Histone+H3+lysine+4+trimethylation+in+sperm+is+transmitted+to+the+embryo+and+associated+with+diet-induced+phenotypes+in+the+offspring%5BTitle%5D)+AND+%22Dev+Cell%22%5BJournal%5D)">Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring.</a> Dev Cell. 56 (5), 671-686.e6 (2021).</li>
<li>Kimmins, S., Sassone-Corsi, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chromatin+remodeling+and+epigenetic+features+of+germ+cells%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Chromatin remodeling and epigenetic features of germ cells.</a> Nature. 434 (7033), 583-589 (2005).</li>
<li>Diaz-Castillo, C., Chamorro-Garcia, R., Shioda, T., Blumberg, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Transgenerational+self-reconstruction+of+disrupted+chromatin+organization+after+exposure+to+an+environmental+stressor+in+mice%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Transgenerational self-reconstruction of disrupted chromatin organization after exposure to an environmental stressor in mice.</a> Sci Rep. 9 (1), 13057(2019).</li>
<li>Gapp, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Implication+of+sperm+RNAs+in+transgenerational+inheritance+of+the+effects+of+early+trauma+in+mice%5BTitle%5D)+AND+%22Nat+Neurosci%22%5BJournal%5D)">Implication of sperm RNAs in transgenerational inheritance of the effects of early trauma in mice.</a> Nat Neurosci. 17 (5), 667-669 (2014).</li>
<li>Chen, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sperm+tsRNAs+contribute+to+intergenerational+inheritance+of+an+acquired+metabolic+disorder%5BTitle%5D)+AND+%22Science%22%5BJournal%5D)">Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder.</a> Science. 351 (6271), 397-400 (2016).</li>
<li>Liberman, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((18S+rRNA+methyltransferases+DIMT1+and+BUD23+drive+intergenerational+hormesis%5BTitle%5D)+AND+%22Mol+Cell%22%5BJournal%5D)">18S rRNA methyltransferases DIMT1 and BUD23 drive intergenerational hormesis.</a> Mol Cell. 83 (18), 3268-3282.e7 (2023).</li>
<li>Tomar, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Epigenetic+inheritance+of+diet-induced+and+sperm-borne+mitochondrial+RNAs%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Epigenetic inheritance of diet-induced and sperm-borne mitochondrial RNAs.</a> Nature. 630 (8017), 720-727 (2024).</li>
<li>Pinhas-Hamiel, O., Zeitler, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+global+spread+of+type+2+diabetes+mellitus+in+children+and+adolescents%5BTitle%5D)+AND+%22J+Pediatr%22%5BJournal%5D)">The global spread of type 2 diabetes mellitus in children and adolescents.</a> J Pediatr. 146 (5), 693-700 (2005).</li>
<li>Galan, C., Krykbaeva, M., Rando, O. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Early+life+lessons%3A+The+lasting+effects+of+germline+epigenetic+information+on+organismal+development%5BTitle%5D)+AND+%22Mol+Metab%22%5BJournal%5D)">Early life lessons: The lasting effects of germline epigenetic information on organismal development.</a> Mol Metab. 38, 100924(2020).</li>
<li>Rando, O. J., Simmons, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((I%27m+eating+for+two%3A+Parental+dietary+effects+on+offspring+metabolism%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">I'm eating for two: Parental dietary effects on offspring metabolism.</a> Cell. 161 (1), 93-105 (2015).</li>
<li>Perez, M. F., Lehner, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intergenerational+and+transgenerational+epigenetic+inheritance+in+animals%5BTitle%5D)+AND+%22Nat+Cell+Biol%22%5BJournal%5D)">Intergenerational and transgenerational epigenetic inheritance in animals.</a> Nat Cell Biol. 21 (2), 143-151 (2019).</li>
<li>Carone, B. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Paternally+induced+transgenerational+environmental+reprogramming+of+metabolic+gene+expression+in+mammals%5BTitle%5D)+AND+%22Cell%22%5BJournal%5D)">Paternally induced transgenerational environmental reprogramming of metabolic gene expression in mammals.</a> Cell. 143 (7), 1084-1096 (2010).</li>
<li>Pastore, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pre-conceptional+paternal+diet+impacts+on+offspring+testosterone+homoeostasis+via+epigenetic+modulation+of+cyp19a1%2Faromatase+activity%5BTitle%5D)+AND+%22NPJ+Metab+Health+Dis%22%5BJournal%5D)">Pre-conceptional paternal diet impacts on offspring testosterone homoeostasis via epigenetic modulation of cyp19a1/aromatase activity.</a> NPJ Metab Health Dis. 2 (1), 8(2024).</li>
<li>Fullston, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Paternal+obesity+initiates+metabolic+disturbances+in+two+generations+of+mice+with+incomplete+penetrance+to+the+F2+generation+and+alters+the+transcriptional+profile+of+testis+and+sperm+microRNA+content%5BTitle%5D)+AND+%22FASEB+J%22%5BJournal%5D)">Paternal obesity initiates metabolic disturbances in two generations of mice with incomplete penetrance to the F2 generation and alters the transcriptional profile of testis and sperm microRNA content.</a> FASEB J. 27 (10), 4226-4243 (2013).</li>
<li>Lumey, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cohort+profile%3A+The+Dutch+Hunger+Winter+Families+Study%5BTitle%5D)+AND+%22Int+J+Epidemiol%22%5BJournal%5D)">Cohort profile: The Dutch Hunger Winter Families Study.</a> Int J Epidemiol. 36 (6), 1196-1204 (2007).</li>
<li>Pembrey, M. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sex-specific%2C+male-line+transgenerational+responses+in+humans%5BTitle%5D)+AND+%22Eur+J+Hum+Genet%22%5BJournal%5D)">Sex-specific, male-line transgenerational responses in humans.</a> Eur J Hum Genet. 14 (2), 159-166 (2006).</li>
<li>Comas-Armangue, G., Makharadze, L., Gomez-Velazquez, M., Teperino, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+legacy+of+parental+obesity%3A+Mechanisms+of+non-genetic+transmission+and+reversibility%5BTitle%5D)+AND+%22Biomedicines%22%5BJournal%5D)">The legacy of parental obesity: Mechanisms of non-genetic transmission and reversibility.</a> Biomedicines. 10 (10), 2461(2022).</li>
<li>Shea, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Genetic+and+epigenetic+variation%2C+but+not+diet%2C+shape+the+sperm+methylome%5BTitle%5D)+AND+%22Dev+Cell%22%5BJournal%5D)">Genetic and epigenetic variation, but not diet, shape the sperm methylome.</a> Dev Cell. 35 (6), 750-758 (2015).</li>
<li>Dupont, J., Bertoldo, M. J., Rak, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Energy+sensors+in+female+and+male+reproduction+and+fertility%5BTitle%5D)+AND+%22Int+J+Endocrinol%22%5BJournal%5D)">Energy sensors in female and male reproduction and fertility.</a> Int J Endocrinol. 2018, 8285793(2018).</li>
<li>Guiton, R., Henry-Berger, J., Drevet, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+immunobiology+of+the+mammalian+epididymis%3A+The+black+box+is+now+open%5BTitle%5D)+AND+%22Basic+Clin+Androl%22%5BJournal%5D)">The immunobiology of the mammalian epididymis: The black box is now open.</a> Basic Clin Androl. 23 (1), 8(2013).</li>
<li>Jarvis, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High+fat+diet+causes+distinct+aberrations+in+the+testicular+proteome%5BTitle%5D)+AND+%22Int+J+Obes%22%5BJournal%5D)">High fat diet causes distinct aberrations in the testicular proteome.</a> Int J Obes. 44 (9), 1958-1969 (2020).</li>
<li>Liu, L. -L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Peroxisome+proliferator-activated+receptor+gamma+signaling+in+human+sperm+physiology%5BTitle%5D)+AND+%22Asian+J+Androl%22%5BJournal%5D)">Peroxisome proliferator-activated receptor gamma signaling in human sperm physiology.</a> Asian J Androl. 17 (6), 942(2015).</li>
<li>Masaki, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long-chain+fatty+acid+triglyceride+metabolism+disorder+impairs+male+fertility%3A+A+study+using+adipose+triglyceride+lipase+deficient+mice%5BTitle%5D)+AND+%22Mol+Hum+Reprod%22%5BJournal%5D)">Long-chain fatty acid triglyceride metabolism disorder impairs male fertility: A study using adipose triglyceride lipase deficient mice.</a> Mol Hum Reprod. 23 (7), 452-460 (2017).</li>
<li>Cheng, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lipidomics+profiles+of+human+spermatozoa%3A+Insights+into+capacitation+and+acrosome+reaction+using+UPLC-MS-based+approach%5BTitle%5D)+AND+%22Front+Endocrinol+%28Lausanne%29%22%5BJournal%5D)">Lipidomics profiles of human spermatozoa: Insights into capacitation and acrosome reaction using UPLC-MS-based approach.</a> Front Endocrinol (Lausanne). 14, 1273878(2023).</li>
<li>Collodel, G., Castellini, C., Lee, J. C. -Y., Signorini, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Relevance+of+fatty+acids+to+sperm+maturation+and+quality%5BTitle%5D)+AND+%22Oxid+Med+Cell+Longev%22%5BJournal%5D)">Relevance of fatty acids to sperm maturation and quality.</a> Oxid Med Cell Longev. 2020, 7038124(2020).</li>
<li>Watkins, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Paternal+diet+programs+offspring+health+through+sperm-+and+seminal+plasma-specific+pathways+in+mice%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+USA%22%5BJournal%5D)">Paternal diet programs offspring health through sperm- and seminal plasma-specific pathways in mice.</a> Proc Natl Acad Sci USA. 115 (40), 10064-10069 (2018).</li>
<li>Darbandi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Oxidative+stress-induced+alterations+in+seminal+plasma+antioxidants%3A+Is+there+any+association+with+keap1+gene+methylation+in+human+spermatozoa%5BTitle%5D)+AND+%22Andrologia%22%5BJournal%5D)">Oxidative stress-induced alterations in seminal plasma antioxidants: Is there any association with keap1 gene methylation in human spermatozoa.</a> Andrologia. 51 (1), e13159(2019).</li>
<li>Deneke, V. E., Pauli, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+fertilization+enigma%3A+How+sperm+and+egg+fuse%5BTitle%5D)+AND+%22Annu+Rev+Cell+Dev+Biol%22%5BJournal%5D)">The fertilization enigma: How sperm and egg fuse.</a> Annu Rev Cell Dev Biol. 37 (1), 391-414 (2021).</li>
<li>Cobellis, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+gradient+of+2-arachidonoylglycerol+regulates+mouse+epididymal+sperm+cell+start-up%5BTitle%5D)+AND+%22Biol+Reprod%22%5BJournal%5D)">A gradient of 2-arachidonoylglycerol regulates mouse epididymal sperm cell start-up.</a> Biol Reprod. 82 (2), 451-458 (2010).</li>
<li>Kawano, N., Yoshida, K., Miyado, K., Yoshida, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lipid+rafts%3A+Keys+to+sperm+maturation%2C+fertilization%2C+and+early+embryogenesis%5BTitle%5D)+AND+%22J+Lipids%22%5BJournal%5D)">Lipid rafts: Keys to sperm maturation, fertilization, and early embryogenesis.</a> J Lipids. 2011, 264706(2011).</li>
<li>Rubinstein, E., Ziyyat, A., Wolf, J., Lenaour, F., Boucheix, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+molecular+players+of+sperm-egg+fusion+in+mammals%5BTitle%5D)+AND+%22Semin+Cell+Dev+Biol%22%5BJournal%5D)">The molecular players of sperm-egg fusion in mammals.</a> Semin Cell Dev Biol. 17 (2), 254-263 (2006).</li>
<li>Satouh, Y., Inoue, N., Ikawa, M., Okabe, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Visualization+of+the+moment+of+mouse+sperm-egg+fusion+and+dynamic+localization+of+IZUMO1%5BTitle%5D)+AND+%22J+Cell+Sci%22%5BJournal%5D)">Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.</a> J Cell Sci. 125 (pt 3), 498-506 (2012).</li>
<li>Kim, J., Seo, S., Kim, T. -Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Metabolic+deuterium+oxide+%28D2O%29+labeling+in+quantitative+omics+studies%3A+A+tutorial+review%5BTitle%5D)+AND+%22Anal+Chim+Acta%22%5BJournal%5D)">Metabolic deuterium oxide (D2O) labeling in quantitative omics studies: A tutorial review.</a> Anal Chim Acta. 1242, 340722(2023).</li>
<li>Li, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sustained+high+glucose+intake+accelerates+type+1+diabetes+in+NOD+mice%5BTitle%5D)+AND+%22Front+Endocrinol+%28Lausanne%29%22%5BJournal%5D)">Sustained high glucose intake accelerates type 1 diabetes in NOD mice.</a> Front Endocrinol (Lausanne). 13, 1037822(2022).</li>
<li>Byers, S. L., Wiles, M. V., Dunn, S. L., Taft, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+estrous+cycle+identification+tool+and+images%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Mouse estrous cycle identification tool and images.</a> PLoS One. 7 (4), e35538(2012).</li>
<li>Katz, J. J. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aKatz%2C+JJ+%22Chemical+and+biological+studies+with+deuterium%22">Chemical and biological studies with deuterium</a>. , Sigma Xi, The Scientific Research Honor Society. (2024).</li>
<li>Oakberg, E. F., Hughes, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Deuterium+oxide+effect+on+spermatogenesis+in+the+mouse%5BTitle%5D)+AND+%22Exp+Cell+Res%22%5BJournal%5D)">Deuterium oxide effect on spermatogenesis in the mouse.</a> Exp Cell Res. 50 (2), 306-314 (1968).</li>
<li>Sumiyoshi, M., Sakanaka, M., Kimura, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+intake+of+high-fat+and+high-sucrose+diets+differentially+affects+glucose+intolerance+in+mice%5BTitle%5D)+AND+%22J+Nutr%22%5BJournal%5D)">Chronic intake of high-fat and high-sucrose diets differentially affects glucose intolerance in mice.</a> J Nutr. 136 (3), 582-587 (2006).</li>
<li>Aragno, M., Mastrocola, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dietary+sugars+and+endogenous+formation+of+advanced+glycation+endproducts%3A+Emerging+mechanisms+of+disease%5BTitle%5D)+AND+%22Nutrients%22%5BJournal%5D)">Dietary sugars and endogenous formation of advanced glycation endproducts: Emerging mechanisms of disease.</a> Nutrients. 9 (4), 385(2017).</li>
<li>Wilkinson, D. J., Brook, M. S., Smith, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Principles+of+stable+isotope+research-with+special+reference+to+protein+metabolism%5BTitle%5D)+AND+%22Clin+Nutr+Open+Sci%22%5BJournal%5D)">Principles of stable isotope research-with special reference to protein metabolism.</a> Clin Nutr Open Sci. 36, 111-125 (2021).</li>
<li>Wilkinson, D. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+validation+of+the+application+of+D2O+stable+isotope+tracer+techniques+for+monitoring+day-to-day+changes+in+muscle+protein+subfraction+synthesis+in+humans%5BTitle%5D)+AND+%22Am+J+Physiol+Endocrinol+Metab%22%5BJournal%5D)">A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans.</a> Am J Physiol Endocrinol Metab. 306 (5), E571-E579 (2014).</li>
</ol>

<table><tbody><tr><td>2H2O filtrato, 99,8 atom % 2H</td><td>Sigma Aldrich</td><td>756822</td><td></td></tr><tr><td>Acetone</td><td>Sigma Aldrich</td><td>179124</td><td></td></tr><tr><td>Agilent&nbsp; SIL-8</td><td>Agilent (Santa Clara, CA, U.S.A.)</td><td></td><td></td></tr><tr><td>C16-Ceramide-13C3-15N</td><td>Standard LGC</td><td>TRC-C342770-25MG</td><td></td></tr><tr><td>diclorometano</td><td>Sigma Aldrich</td><td>34856</td><td></td></tr><tr><td>Etere dietilico</td><td>Sigma Aldrich</td><td>673811</td><td></td></tr><tr><td>etanolo</td><td>Sigma Aldrich</td><td>1,11,727</td><td></td></tr><tr><td>HCG (gonadotropina corionica umana,</td><td>Sigma Aldrich</td><td>CG5-1VL</td><td></td></tr><tr><td>L-lisina-&epsilon;-15N cloridrato</td><td>Sigma Aldrich</td><td>608971</td><td></td></tr><tr><td>Maschio e femmina&nbsp; Topi C57BL/6JOlaHsd</td><td>Inotiv&nbsp;</td><td>C57BL/6JOlaHsd</td><td></td></tr><tr><td>metil terz-butil etere (MTBE)</td><td>Sigma Aldrich</td><td>306975</td><td></td></tr><tr><td>MetOH</td><td>Sigma Aldrich</td><td>1,06,035</td><td></td></tr><tr><td>N,O-bis(trimetilsilil(TMS) trifluoroacetammide (BSTFA)</td><td>Supelco</td><td>15238</td><td></td></tr><tr><td>PBS</td><td>Applichem</td><td>Codice A0965-9010</td><td></td></tr><tr><td>PMSG (cavalla gravida' s siero)</td><td>Lee Biosolutions</td><td>493-10</td><td></td></tr><tr><td>piridina</td><td>Sigma Aldrich</td><td>360570</td><td></td></tr><tr><td>Shimadzu GCMS 2010plus&nbsp;</td><td>Shimadzu</td><td></td><td></td></tr><tr><td>Sitostanolo-5,6,22,23-d4</td><td>Sigma Aldrich</td><td>680028</td><td></td></tr><tr><td>trifluoracetato di sodio</td><td>Sigma Aldrich</td><td>1615138</td><td></td></tr><tr><td>SolariX XR 7T</td><td>Daltonici Bruker</td><td></td><td></td></tr><tr><td>TimsTOF Pro Q-TOF&nbsp;</td><td>Daltonici Bruker</td><td></td><td></td></tr><tr><td>Glucosio U13C&nbsp;</td><td>Sigma Aldrich</td><td>389374</td><td></td></tr><tr><td>Ultimate RS3000 UHPLC&nbsp;</td><td>Thermo Fisher Scientifico</td><td></td><td></td></tr><tr><td>Precolonna VanGuard CSHTM (5,0 &volte; 2,1 mm; 1,7 &mu; m, 130 & Aring;)</td><td>Acque</td><td></td><td></td></tr></tbody></table>

marcatura in vivo di isotopi stabili per l'identificazione con spettrometria di massa dei metaboliti paterni trasferiti dallo spermatozoo all'ovocita durante la fecondazione

Stable Isotope In-Vivo Labeling for Mass-Spectrometry Identification of Paternal Metabolites Transferred from Sperm to Oocyte During Fertilization

Al fine di monitorare il trasferimento dei metaboliti dallo spermatozoo all'ovocita, le morule isolate da femmine C57 BL/6J accoppiate con tori di gruppi MARCATI e NON MARCATI vengono raccolte e analizzate mediante Metabolomica basata sulla spettrometria di massa. I metaboliti sono stati estratti come descritto nei passaggi 6-7 e derivatizzati con TMS come descritto nel passaggio 8. È stata eseguita la profilazione metabolomica e lipidomica di morule MARCATE, morule NON MARCATE, ovociti NON MARCATI, spermatozoi MARCATI e...

Marcatura in vivo di isotopi stabili per l'identificazione con spettrometria di massa dei metaboliti paterni trasferiti dallo spermatozoo all'ovocita durante la fecondazione

Paternal contribution to embryo genetics has so far been limited to allelic sequences for decades. A decade of results suggests, instead, that epigenetic ...

stable-isotope-vivo-labeling-for-mass-spectrometry-identification

Research

Ricerca

JoVE Journal

Biology

Biologia

Tecniche di Imaging Ca 2 + Segnalazione di sperma umano

Stabile mediante l'analisi isotopica profilazione di massa gas-cromatografia spettrometria di flusso intermediario metabolica è descritto nel...

Stabile Profiling isotopica di Flux Intermediario Metabolica in fase di sviluppo e per adulti Caenorhabditis elegans</em

Questo protocollo dimostrerà l'estrazione e l'analisi di acidi grassi bioattivi liberi ed esterificati dalle cellule. Gli acidi grassi sono accuratamente quantificati utilizzando la diluizione degli isotopi stabili, la cromatografia liquida chirale, la cattura di elettroni, la ionizzazione chimica atmosferica, il monitoraggio delle reazioni multiple, la spettrometria di massa (SID-LC-ECAPCI-MRM/MS).

Estrazione dei lipidi cellulari per analisi mirate Stabile diluizione isotopica cromatografia liquida-spettrometria di massa

Mutagenesi bisolfito è il gold standard per l'analisi della metilazione del DNA. Il nostro protocollo modificato per l'analisi consente di metilazione del DNA al livello di singola cellula ed è stato specificamente progettato per i singoli ovociti. Può anche essere utilizzato per il clivaggio stadio embrioni.

Singolo ovociti Mutagenesi bisolfito

L'analisi proteomica di qualsiasi tipo di cellula dipende fortemente sia dalla purezza che dal pre-frazionamento del materiale di partenza al fine di de-complessare il campione prima della cromatografia liquida con spettrometria di massa (MS). Utilizzando le tecniche di back-flushing, è possibile ottenere spermatozoi puri dai roditori. Dopo la digestione, i fosfopeptidi possono essere arricchiti utilizzando il TiO2.

Analisi fosfopeptide di roditori Epididymal spermatozoi

Descriviamo i passaggi che permettono il campionamento veloce in situ di una piccola parte di una singola cella con alta precisione e l'invasione minima utilizzando micro-campionamento basato sul capillare, per facilitare la caratterizzazione chimica di uno snapshot di attività metabolica in vivere gli embrioni utilizzando una piattaforma di spettrometria di massa e di elettroforesi capillare su misura singola cella.

Spettrometria di massa di elettroforesi capillare di microsonda per cella singola metabolomica in embrioni di rana Live (Xenopus laevis)

L'approccio di marcatore trofica dell'acido grasso, vale a dire, l'assimilazione degli acidi grassi come intera molecola e trasferimento nel tessuto dei consumatori con no o minori modifiche, è ostacolata da lacune nel metabolismo dell'acido grasso di invertebrati del suolo piccolo. Isotopologue profilatura viene fornito come un prezioso strumento per districarsi tra interazioni trofiche.

Acido grasso 13C Isotopologue profilatura fornisce approfondimenti trasferimento trofico carbonio e metabolismo dei lipidi dei consumatori invertebrati marini

Qui descriviamo una caratterizzazione proteomica basata sulla spettrometria di massa di linee cellulari con destini tissutali noti nell'embrione vertebrato di Xenopus laevis .

Proteomica della spettrometria di massa guidata da livrea cellulare nell'embrione in via di sviluppo (rana)

Qui viene presentato un metodo per lo screening delle strutture lipidiche attraverso la marcatura degli isotopi stabili utilizzando il loro tempo di ritenzione, mobilità e frammentazione.

Tracciamento de novo dei lipidi mediante marcatura isotopica stabile LC-TIMS-TOF MS/MS

Questo video dimostra la preparazione di un ultra-sottile matrice / analita strato per l'analisi dei peptidi e proteine ​​da Matrix-Assisted Laser ionizzazione di desorbimento spettrometria di massa (MALDI-MS).

MALDI Preparazione del campione: il metodo ultra sottile strato

Scopri la marcatura in vivo con isotopi stabili per identificare i metaboliti paterni trasferiti durante la fecondazione. Questo metodo rivela il ruolo epigenetico dello spermatozoo nello sviluppo embrionale precoce.

Marcatura in vivo di isotopi stabili per lidentificazione con spettrometria di massa dei metaboliti paterni trasferiti dallo spermatozoo allovocita durante la fecondazione (Video) | JoVE Journal

Marcatura in vivo di isotopi stabili per l'identificazione con spettrometria di massa dei metaboliti paterni trasferiti dallo spermatozoo all'ovocita durante la fecondazione

Esplora altri video