Name: Elastomeri magnetoreologici microstrutturati per guidare i cambiamenti nellallineamento dei cardiomiociti (Video) | JoVE Journal
Uploaded: 2025-06-10T12:40:00.000+00:00
Duration: 8 min 10 s
Description: Scopri come i cardiomiociti neonatali di ratto rispondono ai cambiamenti dinamici di rigidità usando elastomeri magnetoreologici microstrutturati. Questa piattaforma semplice e reversibile aiuta a studiare la memoria meccanica cardiaca.

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</ol>

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elastomeri magnetoreologici microstrutturati per guidare i cambiamenti nell'allineamento dei cardiomiociti

Micropatterned Magneto-Rheological Elastomers to Drive Changes in Cardiomyocyte Alignment

Elastomeri magnetoreologici microstrutturati per guidare i cambiamenti nell'allineamento dei cardiomiociti

Substrate-associated cues, such as mechanical and topographic, profoundly influence cellular response. However, much of the foundational research employs ...

micropatterned-magneto-rheological-elastomers-to-drive-changes

Research

Ricerca

JoVE Journal

Bioengineering

Bioingegneria

In questo protocollo, dimostriamo la fabbricazione di substrati di coltura cellulare cardiaca biomimetica realizzati con due materiali polimerici distinti utilizzando la litografia a forza capillare.  I metodi descritti forniscono una tecnica scalabile ed economica per ingegnerizzare la struttura e la funzione dei tessuti cardiaci macroscopici per applicazioni in vitro e in vivo.

Capillare Forza litografia per Cardiac Tissue Engineering

La generazione di allineato tessuto miocardico è un requisito fondamentale per adattare i recenti progressi nel campo della biologia delle cellule staminali a fini clinicamente utili. Qui si descrive un metodo di stampa per microcontatto il controllo preciso di forma e funzione cellulare. Utilizzo di popolazioni altamente purificate di cellule staminali embrionali derivate progenitori cardiaci, abbiamo quindi generare anisotropo tessuto funzionale del miocardio.

Generazione di Allineati tessuto funzionale del miocardio attraverso la stampa microcontatto

Vi presentiamo un nuovo idrogel di poliacrilamide, chiamato idrossi-PAAM, che permette un legame diretto di proteine ​​ECM con un costo minimo o esperienza. La combinazione di idrossi-PAAM Idrogel con stampa microcontact facilita il controllo indipendente di molti spunti del microambiente naturale delle cellule per studiare mechanostransduction cellulare.

Preparazione di idrossi-PAAM idrogel per dissociare gli effetti di meccanotrasduzione Cues

Questo protocollo descrive un nuovo metodo per creare un modello ad elementi finiti spazialmente dettagliate dell'architettura intracellulare dei cardiomiociti da microscopia elettronica e immagini di microscopia confocale. Il potere di questo modello spazialmente dettagliato è dimostrato mediante studi di caso nella segnalazione di calcio e bioenergetica.

Creazione di un modello geometrico strutturalmente realistico agli elementi finiti di un Cardiomyocyte per studiare il ruolo dell'architettura cellulare in biologia dei sistemi del Cardiomyocyte

Qui presentiamo un protocollo per la formazione di una popolazione delle cellule usando stimoli elettrici e meccanici, emulando la fisiologia cardiaca. Questa stimolazione elettromeccanica migliora il potenziale di cardiomiogenici delle cellule trattate ed è una strategia promettente per ulteriore terapia cellulare, la modellazione di malattia e lo screening di stupefacenti.

Simultanea stimolazione elettrica e meccanica per migliorare il potenziale di cardiomiogenici delle cellule

Uno scaffold bioinspired è fabbricato con una tecnica di fotolitografia morbida utilizzando idrogel sagomati dal punto di guida ed elettricamente conduttivi. Gli idrogel micromodellati forniscono l'allineamento delle cellule cardiomiociti cardiomiociti direzionali, con conseguente direzione di azionamento su misura. I microelettrodi flessibili sono integrati anche nello scaffold per portare la controllabilità elettrica per un tessuto cardiaco auto-octuante.

Robot morbido bioispirato con microelettrodi incorporati

Questo protocollo fornisce metodi dettagliati che descrivono la fabbricazione e l'implementazione di una piattaforma di regolazione del carico di carico magnetica per tessuti cardiaci ingegnerizzati.

Regolazione magnetica del postcarico nei tessuti cardiaci ingegnerizzati

Questo protocollo introduce un metodo di micromodellazione senza litografia che è semplice e accessibile a coloro che hanno un background di bioingegneria limitato. Questo metodo utilizza stencil personalizzati tagliati al laser per micropattern proteine a matrice extracellulare in una forma di interesse per la modulazione delle morfologie cellulari. La procedura per la micropatterning è dimostrata utilizzando cardiomiociti derivati da cellule staminali pluripotenti indotti.

Micropatterning a cella singola semplice senza litografia con stencil laser-cut

L'attuale protocollo genera tessuti cardiaci ingegnerizzati a forma di maglia contenenti cellule cardiovascolari derivate da cellule staminali pluripotenti indotte dall'uomo per consentire lo studio della terapia dell'impianto cellulare per le malattie cardiache.

Preparazione di tessuti cardiaci ingegnerizzati a forma di mesh derivati da cellule iPS umane per la riparazione miocardia in Vivo

Questo protocollo mira a descrivere una nuova metodologia per misurare la velocità di sparo cardiaca intrinseca utilizzando la registrazione di array di microelettrodi dell'intero tessuto del nodo senoatriale per identificare i difetti di pacemaking nei topi. Gli agenti farmacologici possono anche essere introdotti in questo metodo per studiare i loro effetti sul pacemaking intrinseco.

Registrazione di microelettrodi della velocità di attivazione dei nodi senoatriali per identificare i difetti intrinseci del pacemaking cardiaco nei topi

Questo lavoro presenta un protocollo flessibile per l'utilizzo della tecnologia FLECS (fluorescently labeled elastomeric contractible surfaces) in formato microwell per una quantificazione semplificata e hands-off delle forze contrattili a singola cellula basata sugli spostamenti visualizzati di micropattern proteici fluorescenti.

Analisi semplificata e ad alta produttività della contrattilità a cella singola utilizzando elastomeri micropatterati

Scopri come i cardiomiociti neonatali di ratto rispondono ai cambiamenti dinamici di rigidità usando elastomeri magnetoreologici microstrutturati. Questa piattaforma semplice e reversibile aiuta a studiare la memoria meccanica cardiaca.

Elastomeri magnetoreologici microstrutturati per guidare i cambiamenti nell'allineamento dei cardiomiociti

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