Name: Somministrazione di δ 9-tetraidrocannabinolo (THC) in topi adolescenti e adulti (Video) | JoVE Journal
Uploaded: 2025-08-01T13:40:00.000+00:00
Duration: 7 min 51 s
Description: Scopri come somministrare THC a topi adolescenti e adulti per studiare gli effetti sul sistema endocannabinoide. Questo protocollo valuta la catalessia, l'attività locomotoria e la risposta al dolore in modelli murini.

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<li>Lim, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adolescent+exposure+to+low-dose+delta9-tetrahydrocannabinol+depletes+the+ovarian+reserve+in+female+mice%5BTitle%5D)+AND+%22Toxicol+Sci%22%5BJournal%5D)">Adolescent exposure to low-dose delta9-tetrahydrocannabinol depletes the ovarian reserve in female mice.</a> Toxicol Sci. 193 (1), 31-47 (2023).</li>
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</ol>

<table><tbody><tr><td>Siringhe da 1 mL TB</td><td>BD</td><td>309659</td><td>200/cassa; Sterile, monouso.</td></tr><tr><td>Fiale di vetro da 8 ml</td><td>Fisher Scientific&nbsp;</td><td>W224584</td><td>144/Cassa, utilizzato per preparare la soluzione iniettabile di THC.</td></tr><tr><td>Software ANY-maze</td><td>QUALSIASI-labirinto</td><td>6000</td><td>Utilizzato per registrare video di test comportamentali e quantificare gli elementi dei test per l'analisi dei dati.</td></tr><tr><td>C57BL/6J Mouse</td><td>Laboratori Jackson</td><td>#000664</td><td>Topi wild type utilizzati per tutti gli esperimenti; Può specificare l'età esatta e il sesso desiderato.</td></tr><tr><td>Bilancia Compass CX</td><td>Ohaus</td><td>CR221</td><td>Utilizzato per pesare i topi per determinare la quantità di THC da iniettare; 220 g massimo, d = 0,1 g.</td></tr><tr><td>Corning Pyroceram Piastra riscaldante</td><td>Fisher Scientifico</td><td>11500151</td><td>Utilizzato per riscaldare il bagno d'acqua per il test Tail Flick.</td></tr><tr><td>Delta-9-tetraidrocannabinolo</td><td>Cayman Chemical&nbsp;</td><td>12068</td><td>Conservato nella cassaforte chiusa a chiave per i farmaci della Tabella 1 in un -30 &ordm; C congelatore.</td></tr><tr><td>Fisherbrand Guanti in nitrile</td><td>Fisher Scientifico</td><td>19-181-610</td><td>100/confezione; Guanti monouso, privi di lattice, utilizzati per dispositivi di protezione individuale adeguati.</td></tr><tr><td>GraphPad Prism v10.4</td><td>GraphPad</td><td>n/a</td><td>Software di analisi statistica utilizzato per analizzare i dati sperimentali ed eseguire test statistici.</td></tr><tr><td>Bicchiere per impieghi gravosi</td><td>Fisher Scientifico</td><td>S81280</td><td>Utilizzato per trattenere l'acqua calda per il test Tail Flick.</td></tr><tr><td>Azoto gassoso ad alta purezza</td><td>Aria gas</td><td>UN1066</td><td>&nbsp; Utilizzato per far evaporare l'etanolo dalla soluzione madre di THC.</td></tr><tr><td>Webcam Logitech C920</td><td>Logitech</td><td>960-001055</td><td>Utilizzato per registrare test comportamentali sugli animali; Carl Zeiss 1080p/30 fps HD USB.</td></tr><tr><td>Apri la casella del campo</td><td>Stoelting Co</td><td>60101</td><td>Scatola con lati opachi per condurre prove in pieno campo; Fianchi facilmente rimovibili per la pulizia.</td></tr><tr><td>Tappetini per l'addestramento degli animali domestici</td><td>Felice</td><td>B088G1N2H8</td><td>85/confezione; 22 pollici e acuto; 22 pollici; utilizzato per avvolgere e tenere i topi per il test Tail Flick.</td></tr><tr><td>Aghi PrecisionGlide</td><td>BD</td><td>305109</td><td>100/cassa; 0,4 mm &acuto; 13 millimetri; Sterile, monouso.</td></tr><tr><td>Salino</td><td>Fisher Scientific (Cytiva)</td><td>Z1377</td><td>Bottiglie da 1 L, grado sterile USP.</td></tr><tr><td>SCOE 1x</td><td>BioFOG</td><td>R-SK</td><td>Soluzione detergente utilizzata per pulire i materiali per i test comportamentali.</td></tr><tr><td>Interpolazione-80</td><td>Sigma Aldrich</td><td>P8074-500ML</td><td>BioXtra, liquido viscoso; CAS # 9005-65-6.</td></tr><tr><td>Bagno d'acqua ad ultrasuoni</td><td>Fisher Scientific (Branson)</td><td>CPX2800</td><td>Controllo della temperatura; Pulitore digitale ad ultrasuoni.</td></tr><tr><td>Miscelatore a vortice</td><td>Fisher Scientifico</td><td>2215365</td><td>Utilizzato per miscelare il contenuto della soluzione iniettabile di THC durante la preparazione.</td></tr></tbody></table>

somministrazione di δ 9-tetraidrocannabinolo (thc) in topi adolescenti e adulti

Administration of &#916;9-Tetrahydrocannabinol (THC) in Adolescent and Adult Mice

Somministrazione di δ 9-tetraidrocannabinolo (THC) in topi adolescenti e adulti

&#916;9-tetrahydrocannabinol (THC) is responsible for the intoxicating (psychotropic) effects of cannabis and cannabis-derived products currently ...

administration-9-tetrahydrocannabinol-thc-adolescent-adult

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Modulazioni ambientali del numero di Mesencefalo dopamina neuroni in topi adulti

Questo protocollo ha lo scopo di consentire ai ricercatori di condurre esperimenti progettati per testare questi aspetti della dipendenza utilizzando la preferenza di luogo condizionata e i saggi di sensibilizzazione comportamentale locomotoria.

Valutazione della cocaina indotta sensibilizzazione comportamentale e conditioned place preference nei topi

Il potenziale vanilloide di tipo 1 (TRPV1) nella regione sovraspinale è stato suggerito di svolgere alcuni ruoli nella funzione cerebrale. Descritto qui è un protocollo per l'iniezione intracerebroventricolare di resiniferatoxin per la desecizione trPV1 sovraspinale nei topi. Vengono inoltre presentate le procedure per alcuni esami del dolore.

Trattamento intracerebroventricolare con Resiniferatoxin e test del dolore nei topi

Qui vi presentiamo il protocollo sforzo delicato cronico imprevedibile nei topi. Questo protocollo induce un fenotipo simile a depressione a lungo termine e permette di valutare l'efficacia degli antidepressivi putativi a invertire i deficit di tipo di depressiva comportamentistici e neuromolecular.

Il protocollo di sforzo delicato cronico imprevedibile per indurre anedonia in topi

Abbiamo sviluppato un modello di stress da sconfitta sociale accelerata per topi adolescenti C57BL/6, che funziona sia nei maschi che nelle femmine e consente l'esposizione durante periodi discreti dell'adolescenza. L'esposizione a questo modello induce l'evitamento sociale, ma solo in un sottogruppo di topi maschi e femmine sconfitti.

Modello di stress di sconfitta sociale per topi maschi e femmine adolescenti C57BL / 6

Qui, descriviamo la somministrazione di farmaci guidata da micropipette (MDA) come un metodo alternativo alla sonda gastrica orale che incentiva l'animale da ricerca a ingerire prontamente i trattamenti con il minimo stress e disagio.

Uso della somministrazione di farmaci guidata da micropipette come metodo alternativo alla sonda gastrica orale nei modelli di roditori

Le cartucce per vaporizzatori di distillato di cannabis sono dispositivi alimentati a batteria che aerosolizzano estratti contenenti alte concentrazioni di cannabinoidi. L'assenza di modelli preclinici consolidati per questi prodotti crea sfide nello studio dei loro effetti fisiologici. Per colmare questa lacuna, è stato sviluppato un modello murino standardizzato di inalazione preclinica per distillati di cannabis vaporizzati.

Sviluppo di un modello di inalazione preclinica per testare i distillati di cannabis vaporizzati

La somministrazione orale tramite sonda gastrica nei roditori può indurre angoscia associata alla contenzione. Abbiamo esplorato la somministrazione oromucosa/buccale di estratto di cannabis arricchito con CBD, in cui i ratti sono stati trattati per 15 giorni con l'estratto a 3 mg/kg/giorno. I trattamenti sono stati somministrati per via orale con una micropipetta, utilizzando una tecnica di tocco affettuoso per maneggiare gli animali.

Mucosa orale come metodo alternativo per la somministrazione di prodotti a base di cannabis nei roditori

Poiché le restrizioni legislative cambiano, con conseguente aumento dell'accessibilità dei prodotti a base di cannabinoidi, è di fondamentale importanza sviluppare modelli per studiare l'impatto di queste esposizioni, in particolare durante la gravidanza. È stato sviluppato un modello preclinico di esposizione prenatale moderata ai cannabinoidi attraverso l'ingestione volontaria, che consente indagini più approfondite.

Modello preclinico di esposizione prenatale al delta-9-tetraidrocannabinolo per valutarne l'impatto sugli esiti dello sviluppo neurologico

Scopri come somministrare THC a topi adolescenti e adulti per studiare gli effetti sul sistema endocannabinoide. Questo protocollo valuta la catalessia, l'attività locomotoria e la risposta al dolore in modelli murini.

Somministrazione di δ 9-tetraidrocannabinolo (THC) in topi adolescenti e adulti

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