Name: Sviluppo di nanovescicole mesenchimali avvolte nella membrana delle cellule staminali per un migliorato apporto genico (Video) | JoVE Journal
Uploaded: 2026-02-17T14:40:00.000+00:00
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Description: Scopri come preparare nanovescicole derivate da cellule staminali mesenchimali che incapsulano vettori AAV per una terapia genica migliorata. Questo metodo offre maggiore efficienza di consegna e resa produttiva scalabile.

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<ol><li>Wang, D., Tai, P. W. L., Gao, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adeno-associated+virus+vector+as+a+platform+for+gene+therapy+delivery%5BTitle%5D)+AND+%22Nat+Rev+Drug+Discov%22%5BJournal%5D)">Adeno-associated virus vector as a platform for gene therapy delivery.</a> Nat Rev Drug Discov. 18 (5), 358-378 (2019).</li>
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<li>Tian, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biomimetic+mesenchymal+stem+cell+membrane-coated+nanoparticle+delivery+of+mkp5+inhibits+hepatic+fibrosis+through+the+Ire%2Fxbp1+pathway%5BTitle%5D)+AND+%22J+Nanobiotechnology%22%5BJournal%5D)">Biomimetic mesenchymal stem cell membrane-coated nanoparticle delivery of mkp5 inhibits hepatic fibrosis through the Ire/xbp1 pathway.</a> J Nanobiotechnology. 22 (1), 741(2024).</li>
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<li>Liang, Y., Lehrich, B. M., Zheng, S., Lu, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Emerging+methods+in+biomarker+identification+for+extracellular+vesicle-based+liquid+biopsy%5BTitle%5D)+AND+%22J+Extracell+Vesicles%22%5BJournal%5D)">Emerging methods in biomarker identification for extracellular vesicle-based liquid biopsy.</a> J Extracell Vesicles. 10 (7), e12090(2021).</li>
<li>Verma, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Seroprevalence+of+adeno-associated+virus+neutralizing+antibodies+in+males+with+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Hum+Gene+Ther%22%5BJournal%5D)">Seroprevalence of adeno-associated virus neutralizing antibodies in males with Duchenne muscular dystrophy.</a> Hum Gene Ther. 34 (9-10), 430-438 (2023).</li>
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</ol>

<table><tbody><tr><td>Sistema di imaging bioluminescenza</td><td>PerkinElmer</td><td>IVIS Spectrum optical imaging system (Lumina III)&nbsp;</td><td></td></tr><tr><td>Sale di potassio di D-Luciferina</td><td>Yeasen Biotechnology</td><td>40902ES03</td><td></td></tr><tr><td>Siero fetale bovino&nbsp;</td><td>Yeasen Biotechnology</td><td>40130ES76</td><td></td></tr><tr><td>Microscopio a fluorescenza</td><td>Zeiss&nbsp;</td><td>Axio Observer 7</td><td></td></tr><tr><td>Griglia di rame rivestita di Formvar/carbonio 200 mesh</td><td>Millipore-Sigma</td><td>TEM-FCF400CU</td><td></td></tr><tr><td>Glutaraldeide&nbsp;</td><td>Millipore-Sigma</td><td>354400</td><td></td></tr><tr><td>HBSS&nbsp;</td><td>Gibco</td><td>C14175500BT</td><td></td></tr><tr><td>Hieff Unicon qPCR TaqMan Probe Master Mix</td><td>Yeasen Biotechnology</td><td>11205ES08</td><td></td></tr><tr><td>Medium di Eagle modificato di Dulbecco ad alta concentrazione di glucosio</td><td>Hyclone</td><td>SH30243</td><td></td></tr><tr><td>Centrifugatrice ad alta velocità</td><td>Beckman Coulter</td><td>Avanti J-E</td><td></td></tr><tr><td>Hoechst 33342&nbsp;</td><td>Beyotime</td><td>C1022</td><td></td></tr><tr><td>Strumento NTA</td><td>Malvern Panalytical</td><td>NanoSight NS300&nbsp;</td><td></td></tr><tr><td>Tubo ultrachiaro a parete sottile con apertura superiore per ultracentrifuga&nbsp;</td><td>Beckman Coulter</td><td>344059</td><td></td></tr><tr><td>OptiPrep iodixanol&nbsp;</td><td>Millipore-Sigma</td><td>D1556</td><td></td></tr><tr><td>Paraformaldeide 4%</td><td>Beyotime</td><td>P0099</td><td></td></tr><tr><td>PBS</td><td>Biosharp</td><td>BL302A</td><td></td></tr><tr><td>Penicillina-streptomicina&nbsp;</td><td>Millipore-Sigma</td><td>V900929</td><td></td></tr><tr><td>Strumento qPCR</td><td>Applied Biosystems&nbsp;</td><td>QS6 real-time PCR system&nbsp;</td><td></td></tr><tr><td>Rotore per centrifuga ad alta velocità</td><td>Beckman Coulter</td><td>JA-25.50&nbsp;</td><td></td></tr><tr><td>Rotore SW 41 Ti Swing-bucket per ultracentrifuga&nbsp;</td><td>Beckman Coulter</td><td>331362</td><td></td></tr><tr><td>Microscopio elettronico a trasmissione&nbsp;</td><td>JEOL</td><td>JEM-2100F&nbsp;</td><td></td></tr><tr><td>Ultracentrifuga</td><td>Beckman Coulter</td><td>Optima XE-100</td><td></td></tr><tr><td>Acetato uranile&nbsp;</td><td>Electron Microscopy Sciences</td><td>NC1375332</td><td></td></tr><tr><td>Filtri di membrane in policarbonato Whatman Nuclepore (200 nm)</td><td>Cytiva</td><td>10417004</td><td></td></tr><tr><td>Filtri di membrane in policarbonato Whatman Nuclepore (400 nm)</td><td>Cytiva</td><td>10417104</td><td></td></tr></tbody></table>

sviluppo di nanovescicole mesenchimali avvolte nella membrana delle cellule staminali per un migliorato apporto genico

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Sviluppo di nanovescicole mesenchimali avvolte nella membrana delle cellule staminali per un migliorato apporto genico

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Sviluppo di nanovescicole mesenchimali avvolte nella membrana delle cellule staminali per un migliorato apporto genico

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