Name: Un metodo HPLC-QQQ-MS efficiente e rapido per la quantificazione degli alcaloidi tropani nelle piante medicinali (Video) | JoVE Journal
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Description: Scopri un metodo HPLC-QQQ-MS per la quantificazione rapida degli alcaloidi tropani in Anisodus tanguticus. Questo protocollo analitico offre elevata sensibilità e precisione per il controllo qualità delle piante medicinali tibetane.

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<table><tbody><tr><td>Agilent MassHunter software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 column</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 reversed-phase column, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>Anisodamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Formic Acid</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>freeze dryer</td><td>EYELA, Tokyo, Japan</td><td>FDU-2110</td><td></td></tr><tr><td>High-speed universal grinder</td><td>Taisite Instrument Co., Ltd., Tianjin, China</td><td>6010210100</td><td>FW-100,24,000 rpm, 100 g capacity, 80 mesh</td></tr><tr><td>Hyoscyamine</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Microporous Membrane 0.22&mu;m</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 water system</td><td>MilliporeSigma, Burlington, MA, USA</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Scopolamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

un metodo hplc-qqq-ms efficiente e rapido per la quantificazione degli alcaloidi tropani nelle piante medicinali

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

Separazione cromatografica HPLC-QQQ-MS e identificazione delle TA Un tipico cromatogramma MRM ottenuto dall'analisi di una miscela standard e di un estratto di radice di A. tanguticus è presentato nelle Figure 3 e 4. Nelle condizioni ottimizzate dell'HPLC, tutti e tre gli alcaloidi tropani bersaglio: iociamina, anisodammina e scopolamina, sono stati separati con successo entro 20 minuti, dimostrando ...

Un metodo HPLC-QQQ-MS efficiente e rapido per la quantificazione degli alcaloidi tropani nelle piante medicinali

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

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Una tecnica migliorata per il rilevamento della trimetilammina nella medicina di derivazione animale mediante gascromatografia dello spazio di testa-spettrometria di massa a quadrupolo tandem

Presentiamo un metodo per l'annotazione e la classificazione rapida basata sulla spettrometria di massa (MS) e sulla spettrometria di massa (MS) degli alcaloidi tropanici, utile sia per la dereplicazione preliminare di campioni contenenti tropani che per la scoperta di nuovi alcaloidi per l'isolamento.

Applicazioni della cromatografia liquida Spettrometria di massa tandem nella ricerca sui prodotti naturali: alcaloidi tropanici come caso di studio

Questo protocollo presenta un metodo convalidato di cromatografia liquida-spettrometria di massa a mobilità ionica ad alta risoluzione per determinare la presenza di alcaloidi dell'ergot negli alimenti in conformità con il Regolamento della Commissione (UE) 2023/915 recentemente pubblicato.

Rilevazione di alcaloidi regolati dell'ergot in matrici alimentari mediante cromatografia liquida-spettrometria di mobilità ionica intrappolata-spettrometria di massa a tempo di volo

Dettagli Questo protocollo i passi importanti necessari per la bioconjugation di un cisteina contenente proteine ​​per un maleimmide, tra cui la purificazione dei reagenti, condizioni di reazione, di purificazione e caratterizzazione bioconjugate bioconjugate.

Sintesi di proteine ​​bioconiugati<em&gt; via</em&gt; Chimica cisteina-maleimmide

Descriviamo la polimerizzazione catalitica per inserzione dell'acido 5-norbornene-2-carbossilico e del 5-vinil-2-norbornene per formare polimeri funzionali con una temperatura di transizione vetrosa molto elevata.

Un protocollo semplice ed efficiente per l&#39;inserimento catalitica polimerizzazione di norborneni funzionali

Una procedura dettagliata per la sintesi di un azide 125 I-etichettati e la radiomarcatura di dibenzocyclooctyne (DBCO) -gruppo-coniugati, nanoparticelle d'oro 13-nm dimensioni utilizzando una reazione click senza il rame è descritto.

Un protocollo ottimizzato per la radiomarcatura efficiente di oro nanoparticelle utilizzando un<sup&gt; 125</sup&gt; Azide gruppo I-etichettati protesico

Scopri un metodo HPLC-QQQ-MS per la quantificazione rapida degli alcaloidi tropani in Anisodus tanguticus. Questo protocollo analitico offre elevata sensibilità e precisione per il controllo qualità delle piante medicinali tibetane.

Un metodo HPLC-QQQ-MS efficiente e rapido per la quantificazione degli alcaloidi tropani nelle piante medicinali

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