Name: fermentation pathways without oxygen
Uploaded: 2019-03-11T09:21:11.000Z
Duration: 1 min 29 s
Channel: JoVE

K12 Standards

Tennessee Academic Standards

High School

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

BIO1.LS1.9

Fermentation

発酵とは 酸素なしで 糖を使う嫌気性プロセスのことです 例えば 乳酸が発酵する間に 筋肉が酸素を使い果たすと グルコースは解糖の間に 2つのピルビン酸分子に分解され 2つのATPを生成し 2つのNAD分子をNADHに還元します その後 NADHは ピルビン酸と反応して乳酸を生成し 二酸化炭素は放出されず ATPはもう作られません 実際 好気的呼吸と同量の ATPを産生するには 細胞ははるかに大量の糖を 消費する必要があります NADHが酸化した後 NAD+は解糖に返って そこでATPは グルコースの分解により集められます 人間の場合 酸素が不足しているとき 筋肉細胞は激しい運動の間に このプロセスによってATPを生産できます その後 蓄積された余分な乳酸塩は 血液によって肝臓に運ばれ そこでピルビン酸に戻されます

発酵

真核生物の多くは、生存し適切に機能するために酸素を必要とします。このような生物は、グルコースと酸素を二酸化炭素と水に代謝する好気呼吸で、大量のエネルギーを生み出します。しかし、ほとんどの真核生物は、酸素がなくとも、嫌気性代謝によって、ある程度のエネルギーを作り出せるのです。
 
 好気呼吸は、一連の酸化・還元反応を経て、最終的な電子受容体である酸素を水に還元して終了します。酸素がないと、この反応は起こりません。代わりに、ピルビン酸などの有機分子を最終的な電子受容体として用いることで、細胞は解糖で生成されたNADHを再生させます。このように、有機分子を使ってNADHからNAD+を再生成するプロセスは発酵と呼ばれます。
 
 発酵は、反応の最終生成物によって2種類に分けられます。1）乳酸発酵と2）アルコール発酵です。哺乳類では、ミトコンドリアがなく好気呼吸のできない赤血球や、激しい運動をした骨格筋で乳酸発酵が起こります。また、ヨーグルトに含まれるような特定の細菌でも生じます。この反応では、ピルビン酸とNADHが乳酸とNAD+に変換されます。
 
 アルコール発酵は2段階工程です。第一段階で、ピルビン酸が二酸化炭素とアセトアルデヒドに変換されます。第2段階では、アセトアルデヒドが電子受容体として働き、付随してNADHをNAD+に変えながらエタノールへと還元されます。全体として、アルコール発酵はピルビン酸とNADHをエタノール、二酸化炭素、NAD+に変換します。酵母はアルコール発酵を使って、糖を二酸化炭素とエタノールに変換します。ビールやワインなどのアルコール飲料は、この工程を利用して作られています。

ほとんどの真核生物は、生存し、適切に機能するために酸素を必要とします。そのような生物は、好気性呼吸中に大量のエネルギーを生成します...

Education

JoVE Core

生物学

Cellular Processes

細胞プロセス

Cellular Respiration

細胞呼吸

KEY TERMS AND CONCEPTS

主要用語と概念

fermentation pathways without oxygen

Fermentation is a metabolic pathway that lets cells make energy without oxygen. It begins after glucose is split into two pyruvate molecules during glycolysis. Glycolysis is the same first step used in aerobic respiration.

When oxygen is unavailable, pyruvate does not enter aerobic respiration. Instead, cells convert pyruvate into other organic molecules through pathways that depend on the organism. A key purpose of fermentation is to regenerate NAD+ from NADH so glycolysis can keep running.

During glycolysis, NAD+ becomes NADH. Fermentation transfers electrons from NADH to an organic molecule, such as pyruvate. This reaction produces a new organic molecule and restores NAD+.

The two main fermentation types are lactic acid fermentation and alcohol fermentation. Lactic acid fermentation occurs in red blood cells, which lack mitochondria, and in skeletal muscles during strenuous exercise. It also happens in certain bacteria, including those found in yogurt. In this pathway, pyruvate and NADH are converted to lactic acid and NAD+.

Alcohol fermentation happens in two steps. First, pyruvate is changed into carbon dioxide and acetaldehyde. Then acetaldehyde accepts electrons and is reduced to ethanol, while NADH becomes NAD+. Overall, alcohol fermentation turns pyruvate and NADH into ethanol, carbon dioxide, and NAD+.

Yeasts use alcohol fermentation to convert sugars into carbon dioxide and ethanol. People harness this process to produce alcoholic beverages such as beer and wine. Fermentation shows how cells can keep making energy when oxygen is limited.

Fermentation Pathways Without Oxygen

what is glycolysis?

Glycolysis: How Cells Break Down Glucose

Glycolysis is the first stage of cellular respiration and a key way cells break down glucose to make energy. Cellular respiration converts food energy from macromolecules into ATP, the cell’s main energy currency. Glycolysis also creates intermediate molecules that can feed into other pathways. In this pathway, one six-carbon sugar such as glucose is split into two three-carbon molecules, including pyruvate. The process produces a net gain of two ATP molecules, since four ATP are made and two...

energy-requiring steps of glycolysis

Glycolysis: Glucose Trapping and Splitting

Glycolysis begins in the cytosol, where glucose is prepared for use as energy. This process has two phases: an energy-requiring phase and an energy-releasing phase. The first phase changes glucose into a more reactive, unstable form before it can move into later steps. In the first step, glucose is phosphorylated, which means a phosphate group is added from ATP. This produces glucose 6-phosphate. The added phosphate is negatively charged, so it cannot cross the hydrophobic cell membrane. As a...

energy-releasing steps of glycolysis

Glycolysis: ATP and NADH Formation

Glycolysis produces ATP and NADH during its energy-releasing phase. These reactions are the last steps of glycolysis and convert glyceraldehyde-3-phosphate, or G3P, into pyruvate. The phase is often described as steps 6 to 10, and it happens twice because two 3-carbon sugars are made earlier in glycolysis. The sixth step begins with two linked events. G3P is oxidized, which means it loses hydrogen, and it is phosphorylated, which means a phosphate group is added. NAD+ acts as the electron...

pyruvate oxidation

How Pyruvate Becomes Acetyl CoA

Pyruvate is prepared for the next stage of cellular respiration inside the mitochondria. After glycolysis, the charged pyruvate molecules enter the mitochondria by active transport. There, they go through three enzymatic reactions so the energy stored in pyruvate can be used by the cell. First, pyruvate dehydrogenase removes the carboxyl group from pyruvate. This step releases carbon dioxide. Next, the stripped molecule is oxidized, and the electrons are picked up by NAD+ to make NADH. The...

the citric acid cycle

Citric Acid Cycle Energy and Products

The citric acid cycle is a key energy-making pathway in cells. It is also called the Krebs cycle or the TCA cycle. In eukaryotic cells, it takes place in the matrix of mitochondria. In prokaryotic cells, it occurs in the cytoplasm. The cycle starts with acetyl CoA, which enters the pathway after pyruvate oxidation. That earlier step makes two acetyl CoA molecules per glucose molecule. For that reason, the citric acid cycle runs twice for each glucose molecule. The pathway includes eight...

electron transport chains

ATP Production by the Electron Transport Chain

ATP production in the electron transport chain is the final stage of cellular respiration. This stage is part of oxidative phosphorylation, which has two steps: the electron transport chain and chemiosmosis. In eukaryotic cells, the electron transport chain sits in the inner mitochondrial membrane. Its main job is to build a proton gradient. That gradient is then used during chemiosmosis to make ATP. The process also regenerates the electron carriers NAD+ and FAD, which are needed again in...

chemiosmosis

ATP Production by Chemiosmosis

ATP production by chemiosmosis is part of oxidative phosphorylation, the efficient stage of cellular respiration that makes most of the cell’s ATP. Oxidative phosphorylation includes two linked processes: the electron transport chain and chemiosmosis. Together, they help generate adenosine triphosphate, or ATP, the main energy molecule used by cells. The electron transport chain is a series of protein complexes in the inner mitochondrial membrane. These complexes pass electrons through a set...

electron carriers

Electron Carriers in Cellular Respiration

Electron carriers help move electrons through cellular respiration. They act like shuttles that can accept electrons, which means they are reduced, or give electrons away, which means they are oxidized. This changing between oxidized and reduced forms is essential for energy production. As glucose breaks down into carbon dioxide and water, it loses electrons at several stages. Electron carriers pick up those electrons and store them temporarily. They then release the electrons into the...

fermentation

Fermentation is a metabolic pathway that lets cells make energy without oxygen. It begins after glucose is split into two pyruvate molecules during glycolysis. Glycolysis is the same first step used in aerobic respiration. When oxygen is unavailable, pyruvate does not enter aerobic respiration. Instead, cells convert pyruvate into other organic molecules through pathways that depend on the organism. A key purpose of fermentation is to regenerate NAD+ from NADH so glycolysis can keep running. <br...

dietary connections

How Food Becomes ATP

Food becomes ATP through connected steps in cellular respiration. Carbohydrates, proteins, and lipids all feed into these pathways. The body can use foods such as apples, cheese, and guacamole as sources of energy. Carbohydrate digestion starts with enzymes that break complex carbohydrates into simple sugars. Glucose can enter glycolysis directly. Fructose and galactose must first be converted into sugars that are intermediates of the glycolytic pathway. Proteins are digested into amino acids.

introduction to cellular respiration

Cellular Respiration: ATP Production Steps

Cellular respiration is the process cells use to turn energy from food into ATP. ATP, or adenosine triphosphate, stores energy in chemical bonds that can be released quickly when cells need it. Much of the energy released during respiration becomes heat, but some of it is captured to make ATP. This process depends on oxidation-reduction, or redox, reactions. In these reactions, oxidation means electron loss and reduction means electron gain. The electron carriers NAD+ and FAD, along with their...

products of the citric acid cycle

Citric Acid Cycle Output in Cellular Respiration

The citric acid cycle is a key stage of aerobic respiration in cells. It is also called the Krebs cycle or the tricarboxylic acid (TCA) cycle. Plants and animals use this oxygen-requiring pathway as part of cellular respiration. This cycle happens after glycolysis and pyruvate oxidation. Glycolysis makes two pyruvate molecules from each glucose molecule. During pyruvate oxidation, each pyruvate becomes acetyl-CoA, which is the input for the citric acid cycle. That means one glucose molecule...

outcomes of glycolysis

Glycolysis Products in Cellular Respiration

Glycolysis is the first step of cellular respiration and breaks glucose into smaller molecules. Cells use this process to pull energy from glucose over a series of chemical reactions. Cellular respiration can happen with oxygen or without oxygen. In both aerobic and anaerobic cellular respiration, glycolysis makes a net gain of two pyruvate molecules, two NADH molecules, and two ATP molecules. Four ATP molecules are produced, but two ATP molecules are used earlier in the pathway. Glycolysis...

atp yield

How Cellular Respiration Makes ATP

Cellular respiration makes ATP from glucose in several stages. The process usually produces 30 to 32 ATP per glucose molecule. Most of that ATP comes from oxidative phosphorylation and the electron transport chain, while 4 ATP are made earlier, with 2 from glycolysis and 2 from the citric acid cycle. The electron transport chain, or ETC, sits in the inner mitochondrial membrane. It has four main protein complexes and ATP synthase, the enzyme that makes ATP. NADH and FADH2 deliver electrons to...

key terms and concepts

Explore how fermentation helps cells make energy without oxygen, regenerate NAD+, and produce lactic acid or ethanol in high school biology.

Fermentation Pathways Without Oxygen | JoVE K12

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Virginia  Standards of Learning

HIgh School

Life Science

LS.4

LS.4.b

Next Generation Science Standards

HS-LS1-7

Florida Department of Education

SC.912.L.18

SC.912.L.18.6

Texas Essential Knowledge and Skills

BIO.11

BIO.11.A

new-york-state-science-learning-standards

New York State Science Learning Standards

matter-and-energy-in-organisms-and-ecosystems

Matter and Energy in Organisms and Ecosystems

HS-LS1-7.

myJoVE Corporation

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BIO1.LS1.1

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MicroRNAs

siRNA - Small Interfering RNAs

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BIO1.LS1.3

Fermentation Pathways Without Oxygen

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