Centrifugal Evaporator
Centrifugal Evaporator - Related Videos
06:43
Rotary Evaporation to Remove Solvent
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2023
Source: Dr. Melanie Pribisko Yen and Grace Tang — California Institute of Technology Rotary evaporation is a technique most commonly used in organic chemistry to remove a solvent from a higher-boiling point compound of interest. The rotary evaporator, or "rotovap", was invented in 1950 by the chemist Lyman C. Craig. The primary use of a rotovap is to dry and purify samples for downstream applications. Its speed and ability to handle large volumes of solvent make rotary evaporation a preferred...
07:07
Working with Centrifuges
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2023
Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Earth's gravitational force is capable of separating heterogeneous mixtures. However, many systems avert this type of separation owing to the length of time usually required in such cases. Centrifugation is one of the most powerful tools for the separation of heterogeneous mixtures1. It involves the application of centripetal force for sedimentation of the heavier phase, leading to separation of...
01:05
Centrifugation
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2024
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
07:52
An Introduction to the Centrifuge
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2023
The centrifuge is an instrument used in nearly every research lab across the globe. Centrifugation is the process by which a centrifuge is used to separate components of a complex mixture. By spinning laboratory samples at very high speeds, the components of a given mixture are subjected to centrifugal force, which causes more dense particles to migrate away from the axis of rotation and lighter ones to move toward it. These particles can sediment at the bottom of the tube into what’s...
06:39
A High Performance Impedance-based Platform for Evaporation Rate Detection
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Cited by 1 •
2016
This paper presents an impedance-based apparatus for evaporation rate detection of solutions. It offers clear advantages over a conventional weight loss approach: a fast response, high-sensitivity detection, a small sample requirement, multiple sample measurements, and easy disassembly for cleaning and reuse purposes.
FAQs
Reduced pressure lowers the boiling point of the solvent, allowing evaporation to occur under gentler thermal conditions than would otherwise be required. This is important for biological samples because it supports solvent removal while limiting prolonged exposure to higher temperatures. The result is a concentrated sample that remains suitable for subsequent bioengineering analysis or purification workflows.
Rotation creates centrifugal force that helps keep liquid samples controlled during solvent removal. In combination with reduced pressure and heating, it helps limit bumping and splashing, which can otherwise cause sample loss or uneven processing. This physical control is especially useful when concentrating biomolecules or analytes in multiple tubes or microplate wells.
Controlled heating supplies energy for solvent evaporation without relying solely on aggressive or prolonged open heating. Because the process also uses reduced pressure, the solvent can be removed at a lower boiling point. This combination helps concentrate DNA, proteins, metabolites, and assay mixtures while supporting compatibility with sensitive biological materials.
Centrifugal evaporation reduces sample volume in a chamber rather than leaving samples exposed to prolonged open heating. The chamber, rotation, reduced pressure, and controlled heating work together to limit splashing and cross-contamination while removing volatile solvent. This makes the approach useful when reproducible concentration and protection of biological samples are important before analysis or purification.
A basic workflow places liquid samples in compatible tubes or a microplate, loads them into the chamber, and applies rotation, reduced pressure, and controlled heating to remove volatile solvent. After the desired volume reduction, the concentrated material can proceed to analysis or purification. The same general setup supports parallel processing across multiple sample containers.
The method is useful when DNA, proteins, metabolites, or assay mixtures must be concentrated before downstream work. Reducing volume can improve compatibility with later analysis or purification by producing a more concentrated preparation without prolonged open heating. Its ability to process multiple tubes or microplates also supports parallel sample preparation in laboratory workflows.