Electric Cell Sensing
Electric Cell Sensing - Related Videos
12:30
Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
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Cited by 213 •
2014
This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.
03:45
Measuring SMC Contraction In Vitro: A Non-Invasive Method to Evaluate Smooth Muscle Cells Contraction using Electric Cell-Substrate Impedance Sensing
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2025
This video describes the measurement of aortic smooth muscle cell (SMC) contraction using electrical cell-substrate impedance sensing, or ECIS. ECIS measures the locomotion of cells and is a reliable method for measuring contractions.
04:17
Approximate Number Sense Test
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University A common carnival game is to ask people to guess the number of jellybeans packed into a jar. The chances that anyone will get the exact number right are low. But what about the chances that someone will guess 17 or 147,000? Probably even less than the chances of guessing the correct answer; 17 and 147,000 just seem irrational. Why? After all, if the beans cannot be taken out and counted one-at-a-time, how can someone tell that an...
04:53
Electric Cell-Substrate Sensing for Real-Time Evaluation of Metal-Organic Framework Toxicological Profiles
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2023
The following study evaluates the toxicological profile of a selected metal-organic framework utilizing electric cell-substrate impedance sensing (ECIS), a real-time, high-throughput screening technique.
16:40
A New Single Chamber Implantable Defibrillator with Atrial Sensing: A Practical Demonstration of Sensing and Ease of Implantation
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Cited by 5 •
2012
Dual-chamber implantable cardioverter-defibrillators (ICDs) may improve detection of atrial fibrillation as well as differentiation of tachycardias. However, this advantage is undermined by complications associated with the second electrode, which is required in conventional dual chamber devices. Therefore, BIOTRONIK has developed a new electrode called the LinoxSMART S DX that, when used in conjunction with the Lumax DX ICD, offers dual-chamber detection without the risks associated with the...
FAQs
When cultured cells attach to an electrode region and spread across it, they change how the applied electrical field passes through or near the cell-covered area. The resulting shifts in impedance, resistance, or conductance provide quantitative indicators of changing cell coverage and behavior. Tracking these signals over time helps distinguish dynamic changes from a single endpoint measurement.
Impedance, resistance, and conductance offer related but distinct electrical readouts of cell activity. Changes in these measurements can accompany proliferation, migration, attachment, spreading, or altered barrier integrity. Monitoring the selected signal continuously allows investigators to connect electrical changes with the timing and progression of cellular events rather than relying only on observations collected after sampling.
Label-free monitoring avoids requiring fluorescent labels and can follow cells without destructive sampling. This supports repeated measurements from the same cultured population as its behavior changes. For bioengineering assays, that continuity is important because adhesion, migration, barrier changes, cytotoxicity, and drug responses can be evaluated as time-dependent processes instead of inferred from separate endpoint samples.
A typical workflow places cultured cells in an electrode-based system, applies an electrical field through or near the cell layer, and records the resulting signal over time. Investigators then relate changes in impedance, resistance, or conductance to the cellular behavior under study. The workflow therefore combines controlled culture conditions, electrical measurement, and time-resolved interpretation.
The method can be applied to cell adhesion, spreading, proliferation, and migration, as well as changes in epithelial or endothelial barrier integrity. These behaviors produce measurable electrical changes that can be followed during an experiment. Consequently, the same general sensing strategy can support both studies of individual cellular activities and assessments of coordinated changes across a cultured cell layer.
In bioengineering, electrical measurements convert dynamic cell physiology into quantitative assay data. Researchers can use that information to investigate cytotoxicity and drug responses while also examining adhesion, wound healing, or barrier behavior. Because measurements are label-free and real-time, the approach helps connect engineered culture systems with therapeutic development and with the changing functional state of cells.