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Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
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Chapters
- 00:05Title
- 01:23Preparing the Arrays
- 04:19Initiating Data Collection
- 06:17Manipulating the Cells During Data Collection
- 07:52Data Analysis and Modeling of Rb and Alpha
- 08:40Typical Experiment and Analysis
- 11:55Conclusion
This protocol reviews Electric Cell-substrate Impedance Sensing, a method to record and analyze the impedance spectrum of adherent cells for the quantification of cell attachment, proliferation, motility, and cellular responses to pharmacological and toxic stimuli. Detection of endothelial permeability and assessment of cell-cell and cell-substrate contacts are emphasized.
