Sample Holder
Sample Holder - Related Videos
07:55
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
0 Views •
Cited by 4 •
2019
A novel sample holder for macromolecular X-ray crystallography along with a suitable handling protocol is presented. The system allows crystal growth, crystal soaking and in situ diffraction data collection at both, ambient and cryogenic temperature without the need of any crystal manipulation or mounting.
05:41
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
0 Views •
Cited by 2 •
2022
The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.
10:16
Protocol for Microplastics Sampling on the Sea Surface and Sample Analysis
0 Views •
Cited by 186 •
2016
The protocol below describes the methodology for: microplastics sampling on the sea surface, separation of microplastic and chemical identification of particles. This protocol is in line with the recommendations for microplastics monitoring published by the MSFD Technical Subgroup on Marine Litter.
01:18
Sampling Methods: Sample Types
0 Views •
2024
Sampling materials are classified into three main types: solid, liquid, and gas. Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
0 Views •
Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
FAQs
Geometry fixes the sample’s position and shape relative to excitation, detection, reagent access, or sensor contact. That alignment helps measurements compare equivalent regions or conditions, while inconsistent geometry can change signal quality or background levels. During method development, researchers should therefore treat positioning and shape as analytical variables rather than merely mechanical details.
Material selection determines whether the holder suits the specimen and the experimental conditions. It must support the sample while remaining compatible with the required interaction, such as excitation, detection, reagent access, or sensor contact. A suitable material helps preserve measurement reliability, whereas an unsuitable choice may contribute to background or reduce consistency.
A holder’s positioning features can determine how readily a measurement interacts with the specimen. In chemistry, that matters when an experiment requires excitation and detection, access for reagents, or contact with a sensor. Designing around the required interaction helps maintain consistency because each sample encounters the measurement system in a comparable way.
These techniques can impose different requirements for how the specimen is positioned and accessed during measurement. A holder used for spectroscopy may need to support the relevant excitation and detection arrangement, while microscopy or diffraction may require another geometry or interaction path. Selecting the design according to the technique helps control signal quality and measurement comparability.
Researchers should match the holder’s material and design to the sample, experimental conditions, and measurement technique. They should consider the required geometry, the way excitation or detection will occur, and whether reagents or sensors must contact the specimen. This evaluation helps limit background, protect the instrument from contamination, and improve reproducibility.
The specimen is placed in the holder, positioned in the defined geometry required by the instrument, and then exposed to the intended measurement or chemical interaction. Depending on the method, the setup must permit excitation, detection, reagent access, or sensor contact. Maintaining the same arrangement across samples supports reliable comparison of results.
An unsuitable holder can change the measured signal, increase unwanted background, or make sample positioning inconsistent. Its design may also fail to match the required access for excitation, detection, reagents, or sensors. In addition, inadequate compatibility with the experiment can increase contamination concerns. These effects complicate comparisons and weaken analytical reproducibility.
They establish a consistent location and geometry so that samples encounter the observation or measurement system under comparable conditions. When the material and design also suit the technique, differences in signal are more likely to reflect differences among specimens rather than changes in setup. This supports reproducible analysis across spectroscopy, microscopy, and diffraction experiments.