Crispr Gene Drive
Crispr Gene Drive - Related Videos
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
11:53
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
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Cited by 18 •
2017
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this method.
07:27
CRISPR Interference-Based Gene Silencing: A Technique for Targeted Repression of Gene Function in Pathogenic Leptospira
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2025
This video demonstrates a CRISPR interference-based gene silencing in pathogenic Leptospira. Targeted repression of gene expression using a CRISPR/Cas system that blocks mRNA formation helps identify candidate genes for pathological and pharmacological studies.
FAQs
The germline provides the cellular context in which the drive can be copied before reproductive cells contribute genetic material to offspring. Cas9 cuts the matching chromosome, and homology-directed repair uses the drive sequence at that cut site. This copying step is central to increasing transmission across generations rather than limiting the change to a single individual.
RNA guidance directs Cas9 toward a matching chromosome sequence, where the nuclease creates a cut. The subsequent repair process determines whether the drive sequence is copied into that location. Because targeting depends on a matching sequence, gene-drive design must connect the chosen allele with the intended genetic site and account for the possibility of resistance mutations.
Resistance mutations can prevent the targeted chromosome from being cut or copied as intended. If such variants arise, the drive may transmit less efficiently or fail to affect the intended genetic target. Genetics research therefore treats resistance as a key factor in drive design, laboratory testing, and assessments of whether the expected inheritance pattern will persist.
Evaluation combines several activities rather than relying on inheritance observations alone. Researchers consider the drive design, test its behavior in the laboratory, model how it could spread, and conduct risk assessment. Together, these stages examine both genetic performance and broader consequences, helping clarify whether observed results support the proposed application or reveal important limitations.
The approach could support studies of gene function by altering how a chosen allele is inherited across generations. It has also been discussed as a possible way to reduce populations that transmit disease or to control invasive species. These applications depend on understanding transmission, ecological spread, resistance, and containment before any practical use is considered.
Genetics research links molecular design with population-level modeling and risk assessment. Modeling can help examine potential spread beyond the original setting, while risk assessment considers ecological effects, resistance mutations, and containment challenges. This broader context matters because inheritance bias can extend the consequences of a genetic change beyond the organisms directly tested in the laboratory.