Raw 264.7 Cells
Raw 264.7 Cells - Related Videos
05:34
Detection and Isolation of Campylobacter spp. from Raw Meat
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Cited by 3 •
2024
Campylobacter is the leading cause of bacterial foodborne gastroenteritis worldwide. Despite establishments enacting measures to reduce the prevalence throughout their facilities, contaminated products consistently reach consumers. The technique developed over the past twelve years addresses limitations of existing methods for isolating and detecting Campylobacter spp. from raw meat.
08:19
Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
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Cited by 1 •
2025
Accurately quantifying Salmonella in poultry at low levels is a current industrial and regulatory challenge. This protocol describes an MPN assay that enables quantification of Salmonella in raw and ready-to-cook poultry products. This method is fast, sensitive, and aligns with FSIS guidelines, enhancing food safety and supporting public health efforts.
07:36
Analysis of Raw and Processed Cyperi Rhizoma Samples Using Liquid Chromatography-Tandem Mass Spectrometry in Rats with Primary Dysmenorrhea
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Cited by 6 •
2022
Here, a comparative analysis of raw and processed Cyperi rhizoma (CR) samples is presented using ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UPLC-MS/MS) in rats with primary dysmenorrhea. The changes in blood levels of the metabolites and the sample constituents were examined between rats treated with CR and CR processed with vinegar (CRV).
09:12
G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
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Cited by 4 •
2016
Simple methods to detect the selective activation of G proteins by G protein-coupled receptors remain an outstanding challenge in cell signaling. Here, Fӧrster resonance energy transfer (FRET) biosensors have been developed by pairwise tethering a GPCR to G protein peptides to probe conformational changes at controlled concentrations in live cells.
10:41
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
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Cited by 8 •
2014
Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.
FAQs
Microbial components are recognized through pattern-recognition receptors on or in the macrophage-like cells. This recognition can activate signaling pathways such as NF-κB, which regulates inflammatory responses and supports cytokine production. The model therefore connects an external infection-related stimulus with measurable changes in innate immune activity, helping investigators examine how host cells respond to pathogen-associated signals.
Phagocytosis provides a functional readout of macrophage behavior alongside inflammatory signaling. When RAW 264.7 cells encounter relevant targets, investigators can examine their capacity to engulf them and relate that activity to broader host responses. Assessing phagocytosis together with cytokine production or pathway activation helps distinguish cellular uptake from the inflammatory processes triggered by microbial recognition.
Different experimental stimuli can produce different patterns of innate immune activity. Microbial components may engage pattern-recognition receptors and promote inflammatory signaling, whereas immunomodulatory compounds can alter those cellular responses. By comparing treated and stimulated conditions, researchers can evaluate effects on phagocytosis, cytokine production, and pathways such as NF-κB, linking a tested compound to specific immune outcomes.
Their reproducible growth and accessible experimental handling allow investigators to examine cellular mechanisms in a practical, controlled model. Researchers can first investigate pathogen interactions, macrophage activation, inflammatory signaling, or compound effects in RAW 264.7 cells, then use the resulting observations to inform studies in primary cells or animal systems. This staged approach supports preliminary evaluation before more complex models.
A typical study exposes the cells to a pathogen-related stimulus, such as a microbial component, or to a candidate antimicrobial or immunomodulatory compound. Investigators then evaluate relevant outcomes, including phagocytosis, inflammatory signaling, or cytokine production. Comparing these responses across experimental conditions can reveal how the stimulus affects macrophage activity and host-pathogen interactions.
The model supports studies of how macrophage-like cells interact with pathogens, become activated, and produce inflammatory mediators. It can also be used to evaluate whether antimicrobial or immunomodulatory compounds alter these responses. Such experiments generate cellular evidence about innate immune mechanisms, pathogen effects, and treatment-related changes before investigators extend the work to primary-cell or animal studies.