Bone Marrow Progenitor Cells
Bone Marrow Progenitor Cells - Related Videos
09:53
Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
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Cited by 17 •
2016
Here we describe an optimized, highly reproducible protocol to isolate Mesodermal Progenitor Cells (MPCs) from human bone marrow (hBM). MPCs were characterized by flow cytometry and nestin expression. They showed the ability to give rise to exponentially growing MSC-like cell cultures while retaining their angiogenic potential.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
09:08
Retroviral Transduction of Bone Marrow Progenitor Cells to Generate T-cell Receptor Retrogenic Mice
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Cited by 11 •
2016
We present a rapid and flexible protocol for a single T cell receptor (TCR) retroviral-based in vivo expression system. Retroviral vectors are used to transduce bone marrow progenitor cells to study T cell development and function of a single TCR in vivo as an alternative to TCR transgenic mice.
03:56
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
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2023
This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic biomarker.
12:54
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
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Cited by 19 •
2014
The nature of the interactions between hematopoietic stem and progenitor cells (HSPCs) and bone marrow niches is poorly understood. Custom hardware modifications and a multi-step acquisition protocol allow the use of two-photon and confocal microscopy to image ex vivo labeled HSPCs homed within bone marrow areas, tracking interactions and movement.
FAQs
Signals from the bone marrow microenvironment help determine whether progenitor cells proliferate, survive, or differentiate. Their effects influence the balance between continued blood-cell production and the formation of particular myeloid or lymphoid lineages. Studying these cues helps explain how normal hematopoiesis is maintained and how altered marrow conditions may affect blood-cell development.
Hematopoietic stem cells provide the source from which progenitor cells arise, while progenitors represent a stage with more restricted developmental potential. This transition connects long-term blood-forming capacity with the production of specific mature blood-cell lineages. Examining it allows researchers to trace hematopoiesis and identify stages that may be affected in blood disorders.
The developmental direction of these cells depends on signaling cues within the marrow environment and the progenitor’s response to those cues. Signals can support progression toward myeloid or lymphoid lineages while also influencing proliferation and survival. Understanding this regulation is important for interpreting changes in blood production during disease or treatment.
Researchers examine these cells to follow how blood-forming cells progress from hematopoietic stem cells toward mature lineages. Their behavior provides information about proliferation, survival, and differentiation within the marrow. This makes progenitor cells useful for investigating the mechanisms of hematopoiesis and for identifying how normal blood-cell production may become disrupted.
Because progenitor cells occupy a regulated stage of blood-cell development, researchers can use them to examine changes in lineage formation, survival, or proliferation associated with blood disorders. Findings from these studies can clarify how bone marrow function is altered and support evaluation of treatments intended to affect blood production or restore its balance.
Their biology supports several medical areas, including research into hematopoiesis, assessment of treatments that affect bone marrow function, and clinical use of bone marrow transplantation. It also informs regenerative strategies designed to restore blood-cell production. Together, these applications connect cellular developmental mechanisms with approaches for understanding or addressing impaired marrow function.