Fungiform Papillae Collection
Fungiform Papillae Collection - Related Videos
09:39
Technique to Collect Fungiform (Taste) Papillae from Human Tongue
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Cited by 30 •
2010
Knowledge of molecular mechanisms underlying gustatory transduction has recently enjoyed significant advances, largely due to using animal models. However, the wide diversity in taste sensitivity and specificity among mammals warrants studies in human tissue. We describe a biopsy technique to collect living taste cells from the papillae on human tongue.
10:50
Denver Papillae Protocol for Objective Analysis of Fungiform Papillae
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Cited by 47 •
2015
Here we present a protocol to measure fungiform papilla density from digital photographs. This method builds prioritization and objective characteristic metrics into the original descriptive work on fungiform papillae by Miller & Reedy (1990).
08:46
Isolation and Culture of Human Fungiform Taste Papillae Cells
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Cited by 20 •
2012
We aimed to develop a reproducible protocol for isolating and maintaining long-term cultures of human fungiform taste papillae cells. Cells from human fungiform papillae obtained by biopsy were successfully maintained in culture for more than eight passages (12 months) without loss of viability.
03:38
Isolation of Circumvallate Papillae (CVP) Epithelium from Mouse Model: An Enzymatic Procedure to Separate CVP Epithelium from Mouse Tongue
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2025
In this video, we describe the isolation of circumvallate papillae (CVP) epithelium from a murine tongue.
07:40
Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
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Cited by 7 •
2021
This paper describes methods for tissue preparation, staining, and analysis of whole fungiform, circumvallate, and palate taste buds that consistently yield whole and intact taste buds (including the nerve fibers that innervate them) and maintain the relationships between structures within taste buds and the surrounding papilla.
FAQs
These cellular components connect the sampled tissue to taste perception and related sensory functions. Examining them allows investigators to assess how the papillae are organized and maintained rather than studying tongue epithelium as an undifferentiated surface. Their presence also makes the collected tissue relevant to research on epithelial maintenance, sensory biology, and altered taste function.
Collected papillae provide tissue in which investigators can examine cellular organization after disease, medication exposure, aging, or injury. Microscopic, histological, and molecular findings may show changes associated with altered taste function or epithelial maintenance. This makes sampling useful for connecting observable taste changes with structural or molecular features of the anterior tongue.
Nerve interactions are one of the biological relationships that can be examined in collected fungiform papillae. Because these structures contain taste buds and sensory cells, their tissue context can support investigation of how sensory elements relate to surrounding tongue tissue. Such studies contribute to understanding taste perception and the biological responses associated with tissue injury or regeneration.
The papillae offer a localized tissue sample for studying epithelial maintenance and regeneration on the tongue. Researchers can evaluate the organization of the collected tissue and investigate molecular features associated with repair or renewal. This is relevant when examining how injury affects sensory structures and whether tissue changes accompany recovery or altered taste-related function.
The procedure begins by identifying the relevant mushroom-shaped papillae on the anterior lingual surface. Investigators then carefully excise or isolate the selected tissue and preserve it for later analysis. This sequence links anatomical selection with sample integrity, allowing the collected material to remain suitable for microscopic, histological, or molecular investigation.
Collected tissue can be examined microscopically, processed for histological assessment, or analyzed with molecular methods. These approaches provide complementary information: microscopy and histology support evaluation of cellular and tissue organization, while molecular analysis can examine biological features at the molecular level. Together, they help relate tissue characteristics to sensory function, maintenance, or regeneration.
Researchers may use these samples to investigate taste perception, epithelial maintenance, nerve interactions, and tissue regeneration. In medical research, the approach is especially relevant when studying how disease, medications, aging, or injury alter taste function or tongue organization. The resulting tissue analyses can connect clinical or biological changes with local cellular features.