Journal
/
Neuroscience
/
Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds

DOI:
10.3791/62126-v

•

07:40 min

•

February 11, 2021

•

,
1Anatomical Sciences and Neurobiology,University of Louisville

Chapters

  • 00:04Introduction
  • 01:44Tissue Preparation
  • 04:23Results: Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describes methods for tissue preparation, staining, and analysis of whole fungiform, circumvallate, and palate taste buds that consistently yield whole and intact taste buds (including the nerve fibers that innervate them) and maintain the relationships between structures within taste buds and the surrounding papilla.

Tags

Keywords: Whole-mount StainingTaste BudsFungiformCircumvallatePalateLingual EpitheliumInnervationTransducing CellsOCT Compound

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62126?article_type=v

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos
check_url/62126?article_type=v

Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
check_url/62126?article_type=v

Reproducible Mouse Sciatic Nerve Crush and Subsequent Assessment of Regeneration by Whole Mount Muscle Analysis
check_url/62126?article_type=v

Isolation and Culture of Human Fungiform Taste Papillae Cells
check_url/62126?article_type=v

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation
check_url/62126?article_type=v

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
check_url/62126?article_type=v

Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
check_url/62126?article_type=v

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna
check_url/62126?article_type=v

Whole-mount Imaging of Mouse Embryo Sensory Axon Projections
check_url/62126?article_type=v

Whole Mount Labeling of Cilia in the Main Olfactory System of Mice

Read Article