Platinum Wire Burn

Platinum wire burn is a laboratory sterilization technique that uses direct heat to decontaminate a platinum wire or inoculating loop before and after handling microbial cultures. Passing the wire through a flame raises its temperature sufficiently to destroy bacteria, fungi, and other microorganisms, helping prevent carryover and contamination between samples. In medical microbiology, this practice supports aseptic technique during culture inoculation, isolation of colonies, and preparation of specimens for identification and antimicrobial testing. Proper wire sterilization improves the reliability of diagnostic results and protects laboratory personnel by limiting the spread of viable organisms.

Platinum Wire Burn - Related Videos

Education

JoVE Science Education - Engineering

Hot Wire Anemometry

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2023

Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI Hot-wire anemometers have a very short time-response, which makes them ideal to measure rapidly fluctuating phenomena such as turbulent flows. The purpose of this experiment is to demonstrate the use of hot-wire anemometry.

Research

JoVE Journal - Chemistry

Minimum Burning Pressures of Water-based Emulsion Explosives

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2017

We present an apparatus based on hot-wire ignition in a pressurized enclosure and an associated methodology to measure the minimum pressure required to induce sustained combustion in water-based emulsion explosives. This method improves product characterization to allow one to use them more safely during pumping and mixing operations.

Burn Injuries

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2023

Burn injuries occur when the skin and underlying tissues are damaged due to exposure to heat, electricity, chemicals, radiation, or friction. They can vary in severity, from minor superficial burns to severe deep burns that can be life-threatening. The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...

Establishing a Mouse Model of Burn Infections with Pathogenic Bacteria

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2026

Source: Wang, Y., et al. In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging. J. Vis. Exp. (2017)This video demonstrates how to establish a burn infection in a mouse model by applying a heated brass block followed by pathogenic bacterial inoculum. The bacteria colonize the exposed extracellular matrix and form a biofilm, enabling in vivo studies of host-pathogen interactions.

Direct Mouse Trauma/Burn Model of Heterotopic Ossification

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Cited by 68 •

2015

An Achilles tenotomy and burn injury model of heterotopic ossification allows for the reliable study of trauma induced ectopic bone formation without the application of exogenous factors.

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