Rna Assays
Rna Assays - Related Videos
05:07
RNA-Protein Pull-Down Assay to Isolate RNA-Binding Proteins via Affinity Extraction
0 Views •
2025
This video demonstrates an in vitro RNA pull-down assay to identify RNA-binding proteins (RBPs), which interact with the adenylate-uridylate-rich element (ARE) sequences in mRNA. The target RBPs from a cell lysate are mixed with an RNA probe to form RNA-protein complexes. The complexes are isolated via affinity purification utilizing the affinity of the desthiobiotin label of the RNA probe to a streptavidin-labeled magnetic bead.
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
05:52
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
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Cited by 22 •
2013
A suite of colorimetric assays is described for rapidly distinguishing protein, RNA, DNA, and reducing sugars in potentially heterogeneous biomolecular samples.
06:30
SELEX-Based In Vitro Binding Assay to Identify RNA-Protein Interactions
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2025
In this video, we describe the systematic evolution of ligands by the exponential enrichment (SELEX) method to identify specific RNA-binding sequences for a target protein of interest. The protein is incubated with a large pool of randomized RNA sequences, and the protein-binding RNA sequences are isolated, PCR-amplified, and sequenced to identify their protein-binding sites.
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Cited by 15 •
2016
We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.
FAQs
Assay design determines which stage supplies the most useful evidence. After RNA is isolated, conversion to complementary DNA can prepare transcripts for amplification or sequencing, whereas probe hybridization provides a targeted measurement. The choice among amplification, sequencing, and hybridization therefore depends on whether the study emphasizes transcript quantification, expression changes, or broader RNA characterization.
Complementary DNA, or cDNA, provides an intermediate form of the RNA signal for downstream analysis. In the described workflow, investigators convert isolated RNA into cDNA before amplification or sequencing. This step links the original transcript population to measurements that can be compared across samples, making it useful for examining altered gene activity in neurons, glia, or brain tissue.
Probe-based and sequencing-based readouts answer related but different questions. Hybridization with specific probes focuses measurement on selected transcripts, while sequencing supports characterization of the RNA molecules present. Amplification can also be included as a distinct stage before analysis. Selecting among these approaches shapes whether an experiment prioritizes targeted quantification, expression differences, or broader transcript analysis.
RNA assay results depend partly on which cells contribute the sampled material. Neurons and glial cells may have different gene-regulation patterns, so measurements from brain tissue can reflect a mixture of cellular states. Distinguishing these contexts helps researchers interpret expression changes more accurately when comparing tissue across disease, development, injury, or treatment responses.
A typical workflow begins with RNA isolation from cells or tissue, followed by a selected analysis route. Researchers may convert the material to complementary DNA, amplify it, sequence it, or hybridize it with specific probes. The sequence and combination of these steps should match the intended outcome, such as measuring transcripts, identifying expression changes, or characterizing RNA populations.
RNA assays are useful when researchers ask how gene regulation changes during neural development, after injury, or following treatment. They also support comparisons between healthy and diseased brain tissue and molecular profiling of neurons and glial cells. These applications connect transcript measurements with studies of neural circuits, biomarkers, cellular responses, and disease mechanisms.
Changes in transcript measurements can provide evidence of altered gene activity and molecular responses within neural tissue. Comparing samples across health, disease, development, injury, or treatment can reveal patterns associated with cellular state. These patterns may then support investigations of disease mechanisms, biomarker development, and the molecular regulation underlying neuronal or glial responses.