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Biology

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
 

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Article doi: 10.3791/54090
August 4th, 2016

Summary August 4th, 2016

We describe a targeted RNA sequencing-based method that includes preparation of indexed cDNA libraries, hybridization and capture with custom probes and data analysis to interrogate selected transcripts for gene expression, mutations, and gene fusions. Targeted RNAseq permits cost-effective, rapid evaluation of selected transcripts on a desktop sequencer.

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