Measurement of &#947;HV68 Infection in Mice

Sara Dolatshahi Pirooz; Joo-Hyung Lee; Zhen Zhao; Duojiao Ni; Soohwan Oh; Chengyu Liang

doi:10.3791/3472

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Measurement of γHV68 Infection in Mice

マウスにおけるγHV68感染の測定

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13:22 min

November 22, 2011

DOI: 10.3791/3472-v

Sara Dolatshahi Pirooz¹, Joo-Hyung Lee¹, Zhen Zhao¹, Duojiao Ni¹, Soohwan Oh¹, Chengyu Liang¹

¹Department of Molecular Microbiology and Immunology,University of Southern California, Los Angeles

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Overview

This article presents a protocol for studying 纬-Herpesvirus (纬-HV) infections in mice, focusing on both acute and latent stages. The methods include plaque-forming, infectious center, and qPCR assays to quantify viral load.

Key Study Components

Area of Science

Virology
Infectious Diseases
Animal Models

Background

纬-Herpesviruses establish lifelong infections in hosts.
纬-HV68 serves as a model for studying 纬-HV lifecycle and pathogenesis.
Understanding acute and latent infections is crucial for therapeutic strategies.
In vivo models allow for detailed analysis of viral behavior.

Purpose of Study

To characterize the lifecycle of 纬-HV68 in a mouse model.
To evaluate acute and latent stages of infection.
To provide a protocol for quantifying viral load using various assays.

Methods Used

Intranasal infection of mice with 纬-HV68.
Collection and homogenization of lung tissue for acute viral load assessment.
Harvesting spleen for latent viral load analysis.
Performing plaque and infectious center assays to evaluate viral replication.

Main Results

Acute viral load is determined through plaque assays.
Latent viral load is assessed via infectious center assays.
Results illustrate the spatiotemporal profiles of viral replication.
Findings contribute to understanding 纬-HV pathogenesis in vivo.

Conclusions

The study provides a reliable method for assessing 纬-HV68 infection stages.
Insights gained can inform future research on herpesvirus infections.
Protocols established can be adapted for other 纬-HVs.

Frequently Asked Questions

What is 纬-Herpesvirus?

纬-Herpesvirus is a type of virus that can establish lifelong infections in hosts, often leading to various diseases.

How is the viral load measured in this study?

Viral load is measured using plaque-forming assays and infectious center assays on lung and spleen tissues.

Why use a mouse model for this research?

Mice provide a genetically tractable model to study the lifecycle and pathogenesis of 纬-Herpesviruses in vivo.

What are the stages of infection evaluated?

The study evaluates both acute and latent stages of 纬-HV68 infection.

What is the significance of understanding latent infections?

Understanding latent infections is crucial for developing effective treatments and vaccines against herpesviruses.

Can the methods be applied to other viruses?

Yes, the protocols can be adapted for studying other 纬-Herpesviruses.

γ-ヘルペスウイルス（γ- HVS）生涯その宿主に持続性を確立する。 γ- HV68とマウスの感染症は、遺伝的に扱いやすいが用意されています

このビデオでは、マウスの鼻腔内感染後に、プラークおよび感染性センターアッセイにより、急性および潜伏性ヘルペスウイルス感染症を評価します。最初に生理学的曝露を模倣するために、マウスはニューロンガンマヘルペスウイルスの鼻腔内投与によって感染します。68、5〜7日後、肺組織が収集され、均質化されます。

次いで、急性ウイルス量を決定するためにプラークアッセイが行われる。潜伏ウイルス量を決定するために、マウスから脾臓を採取する。感染後12〜14日後に、感染したSPOCサイトの一つ細胞懸濁液が生成され、感染センターアッセイが行われる。これらのアッセイの結果は、in vivoでのウイルス複製と感染性の時空間プロファイルを明らかにします。

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