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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
JoVE Journal
生物工学
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JoVE Journal 生物工学
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

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10:33 min

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January 08, 2014

DOI:

10.3791/51023-v

DOI:
10.3791/51023-v

•

10:33 min
January 08, 2014

•

1 Views
, , , , , , , ,
1Department of Chemical and Biomolecular Engineering,Ohio University, 2Biomedical Engineering Program,Russ College of Engineering and Technology, Ohio University, 3皮膚学部,Brigham and Women’s Hospital, Harvard Medical School

概要

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Compared with traditional affinity chromatography using protein A agarose bead-packed columns, protein A membrane adsorbers can significantly speed laboratory-scale isolation of antibodies and other Fc fragment-expressing proteins. Appropriate analysis and quantification methods can further accelerate protein processing, allowing isolation/characterization to be completed in one workday, instead of 20+ work hours.

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Cite this Article

Burdick, M. M., Reynolds, N. M., Martin, E. W., Hawes, J. V., Carlson, G. E., Cuckler, C. M., Bates, M. C., Barthel, S. R., Dimitroff, C. J. Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow. J. Vis. Exp. (83), e51023, doi:10.3791/51023 (2014).

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