Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
Cheung, S. T., Shakibakho, S., So, E. Y., Mui, A. L. Transfecting RAW264.7 Cells with a Luciferase Reporter Gene. J. Vis. Exp. (100), e52807, doi:10.3791/52807 (2015).