Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Yoshikazu Kishino; Tomohisa Seki; Shinsuke Yuasa; Jun Fujita; Keiichi Fukuda

doi:10.3791/53225

ジャーナル

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発生生物学

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フィーダーフリー条件にセンダイウイルスを用いてヒト末梢T細胞から人工多能性幹細胞の生成

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

フィーダーフリー条件にセンダイウイルスを用いてヒト末梢T細胞から人工多能性幹細胞の生成

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11,899 Views

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09:32 min

•

November 11, 2015

DOI:

10.3791/53225-v

DOI:

10.3791/53225-v

•

09:32 min

November 11, 2015

•

3 Views

Yoshikazu Kishino¹, Tomohisa Seki², Shinsuke Yuasa¹, Jun Fujita¹, Keiichi Fukuda¹

¹Department of Cardiology,Keio University School of Medicine, ²Department of Emergency and Critical Care Medicine,Keio University School of Medicine

概要

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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Induced Pluripotent Stem Cells IPSCs Peripheral T Cells Sendai Virus Feeder-free Conditions Regenerative Therapies T Cell Receptor Antigen-specific T Cells Genome Rearrangement Clinical Application Fetal Bovine Serum Feeder Cells Biosafety Activated T Cells

フィーダーフリー条件にセンダイウイルスを用いてヒト末梢T細胞から人工多能性幹細胞の生成

概要

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