An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Martin H. M. Sailer; Durga Sarvepalli; Catherine Br&#233;g&#232;re; Urs Fisch; Marin Guentchev; Michael Weller; Raphael Guzman; Bernhard Bettler; Arkasubhra Ghosh; Gregor Hutter

doi:10.3791/54018

ジャーナル

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発生生物学

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創薬に適していEMT調査のための酵素および無血清神経幹細胞培養モデル

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

JoVE Journal
発生生物学

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JoVE Journal 発生生物学

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

創薬に適していEMT調査のための酵素および無血清神経幹細胞培養モデル

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9,979 Views

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07:43 min

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August 23, 2016

DOI:

10.3791/54018-v

DOI:

10.3791/54018-v

•

07:43 min

August 23, 2016

•

9959 Views

Martin H. M. Sailer¹, Durga Sarvepalli², Catherine Brégère³, Urs Fisch³, Marin Guentchev⁴, Michael Weller⁶, Raphael Guzman³, Bernhard Bettler¹, Arkasubhra Ghosh*², Gregor Hutter*⁵

¹Dept. of Biomedicine, Pharmacenter,University of Basel ²Molecular Signalling and Gene Therapy,Narayana Nethralaya Foundation, Narayana Health City ³Brain Ischemia and Regeneration, Department of Biomedicine,University Hospital Basel ⁴Department of Neurosurgery,Klinikum Idar-Oberstein ⁵Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine,Stanford University ⁶Department of Neurology, Laboratory of Molecular Neuro Oncology,University Hospital of Zurich

概要

Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.