Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

Dana Charuvi; Reinat Nevo; Ifat Kaplan-Ashiri; Eyal Shimoni; Ziv Reich

doi:10.3791/54066

ジャーナル

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生物学

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クライオ走査電子顕微鏡により凍結破砕葉組織内の光合成膜の超分子組織を学びます

$Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy$

JoVE Journal
生物学

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JoVE Journal 生物学

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

クライオ走査電子顕微鏡により凍結破砕葉組織内の光合成膜の超分子組織を学びます

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11,937 Views

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13:52 min

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June 23, 2016

DOI:

10.3791/54066-v

DOI:

10.3791/54066-v

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13:52 min

June 23, 2016

•

3 Views

Dana Charuvi^1,3, Reinat Nevo¹, Ifat Kaplan-Ashiri², Eyal Shimoni², Ziv Reich¹

¹Department of Biological Chemistry,Weizmann Institute of Science, ²Department of Chemical Research Support,Weizmann Institute of Science, ³Institute of Plant Sciences,Agricultural Research Organization, Volcani Center

筆記録

概要

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.