マウスと次世代シーケンサーによるひと抗体のレパートリーの同定
14K 回視聴
•
4 件の引用
•
08:51 分
•
2019年3月15日
DOI :10.3791/58804-v
2019年3月15日
•14K 回視聴
Lin Sun1 , Naoko Kono2 , Hiroyuki Toh3 , Hanbing Xue1 , Kaori Sano4,5 , Tadaki Suzuki4 , Akira Ainai4 , Yasuko Orba6 , Junya Yamagishi7,8 , Hideki Hasegawa4,5 , Yoshimasa Takahashi9 , Shigeyuki Itamura2 , Kazuo Ohnishi9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba
ここでは、分析と構造の可視化と全体抗体レパートリーの憲法のためのプロトコルについて述べる。次世代シーケンシングによる抗体 RNA の広大な配列の取得が含まれます。
Chapters in this video
0:04
Title
1:23
Nucleic Acid Isolation from Immune Cells and Tissues
3:04
cDNA Synthesis and PCR Amplification
4:58
NGS Sequencing of Libraries
5:33
Results: Identification of Mouse and Human Antibody Repertoires
8:11
Conclusion
Related Videos
著作権 © 2026 MyJoVE Corporation. 無断転載を禁じます。