8,834 Views
•
13:04 min
March 01, 2019
DOI:
10.3791/59233-v
伝統的な cDNA を用いた発現技術の潜在的な機能を持つ、複数のスプライス フォームによる長鎖非コード Rna 発現限定適用しています。このレビューは、長鎖非コード RNA の複数のスプライスバリアント ノザンに CRISPR 技術を使用してプロトコルを報告します。
11:31
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
関連ビデオ
14065 Views
10:46
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
9280 Views
13:47
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
9511 Views
09:16
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
7510 Views
08:20
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
4085 Views
N/A
Enforced Activation of Enhancer RNAs In Situ through the dCas9 Synergistic Activation Mediator System
2442 Views
11:28
Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection
17563 Views
08:14
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
12154 Views
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
1674 Views
13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
8.8K Views
Read Article
Cite this Article
Rankin, C. R., Treger, J., Faure-Kumar, E., Benhammou, J., Anisman-Posner, D., Bollinger, A. E., Pothoulakis, C., Padua, D. M. Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR. J. Vis. Exp. (145), e59233, doi:10.3791/59233 (2019).
Download .ris file
Copy
Share Video
.