比較薬評価の宿主としてゼブラフィッシュ幼虫を用いた膵臓癌の患者由来異種異種移植片モデル

1:28

Preparing the Equipment for the Microinjection

2:21

Preparing Embryos for Transplantation

2:56

Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue

4:56

Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately

6:29

Injecting Mixed Cell Suspension into the Zebrafish

7:10

Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities

8:11

Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae

9:12

Conclusion