Inducing Apical Periodontitis in Mice

Elisheva Goldman; Eli Reich; Itzhak Abramovitz; Michael Klutstein

doi:10.3791/59521

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Biology

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JoVE Journal Biology

Inducing Apical Periodontitis in Mice

マウスにおけるアピカル歯年理炎の誘導

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10:26 min

August 6, 2019

DOI: 10.3791/59521-v

Elisheva Goldman^1,2, Eli Reich¹, Itzhak Abramovitz*², Michael Klutstein*¹

¹Institute of Dental Sciences, Faculty of Dental Medicine,Hebrew University of Jerusalem, ²Department of Endodontics,Hadassah Ein Kerem Medical Center

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Overview

This study presents a novel protocol for locally inducing apical periodontitis in mice, facilitating controlled investigation of periapical inflammation. The method involves drilling the mouse's tooth to expose pulp and offers various analysis techniques, including micro-CT and histology.

Key Study Components

Research Area

Apical periodontitis
Inflammatory response
Dental pathology

Background

Mouse models are advantageous for dental research.
This protocol provides a cost-effective approach.
Procedure visualization enhances technique understanding.

Methods Used

Drilling and pulp exposure technique
Mice as the biological system
Micro-CT and histological analysis

Main Results

Successful induction of apical periodontitis demonstrated.
Significant inflammation observed post-procedure.
Protocols validated for accuracy in results.

Conclusions

The study effectively demonstrates a method for studying dental inflammation in a mouse model.
Findings have implications for understanding periapical diseases.

Frequently Asked Questions

What is apical periodontitis?

Apical periodontitis is an inflammatory condition affecting the dental pulp and surrounding tissues, typically caused by infection.

Why use mice in this study?

Mice provide an accessible and controlled model for studying complex dental diseases.

What are the main advantages of this protocol?

It is cost-effective, technically simple, and allows for controlled observation of periapical inflammation.

What analysis methods are used?

Micro-CT imaging and histology are utilized to assess inflammation and tissue structure.

How is the protocol validated?

Validation is achieved through consistent observations of induced inflammation and tissue responses.

Can this method be applied to other studies?

Yes, it can be adapted for various dental and pulpal research projects.

What long-term effects are monitored post-procedure?

Animal weight and behavior are monitored for 42 days following the procedure.

ここで、マウスにおいて局所的に頂点歯状炎を誘導するプロトコルを提示する。マウスの歯に穴を開け、パルプを露出させ、局所的な炎症を引き起こす方法を示します。また、マイクロCTや組織学など、この炎症の性質を調べるための分析方法も実証されている。

我々のプロトコルは、アクセス可能で制御されたマウスモデルにおける近腹歯間炎の研究を可能にする。したがって、このプロトコルは、患者のサンプルまたはインビトロモデルよりも利点があります。この技術の主な利点は、マウスが十分に研究されており、方法は施設要件の面で技術的に簡単であり、費用対効果が高いということです。

この手順は、歯の小さな寸法のために困難であるため、技術の位置と性能について学ぶために手順を視覚化することが有益です。つま先ピンチへの応答の欠如を確認した後、閉じたセル押出ポリスチレンフォーム表面上の支配的な手側にマウスを置き、テープで足を固定します。切歯1対1の単一のゴムバンドを使用し、長い針を閉細胞押出ポリスチレンフォーム表面にピン留めし、口を開く。

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