6,388 Views
•
08:03 min
September 19, 2019
DOI:
10.3791/59950-v
ここでは、細胞および脳組織中の5-ヒドロキシメチルシトシンを検出するプロトコルを提示し、免疫蛍光染色およびDNAドットブロット法を利用する。
09:42
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
関連ビデオ
9647 Views
07:19
High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
14992 Views
12:11
Methodology for Accurate Detection of Mitochondrial DNA Methylation
13329 Views
07:50
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
8904 Views
09:51
Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
15252 Views
07:16
Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
1355 Views
10:09
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
7440 Views
06:26
Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
11936 Views
09:44
Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
2934 Views
07:13
Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
7228 Views
Read Article
Cite this Article
Zhuang, Y., Chen, J., Xu, W., Shu, Q., Li, X. The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice. J. Vis. Exp. (151), e59950, doi:10.3791/59950 (2019).
Download .ris file
Copy
Share Video
.