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10:06 min
September 17, 2019
DOI:
10.3791/60242-v
リボソームアフィニティ精製(TRAP)の翻訳により、細胞型特異的翻訳mRNAの迅速かつ効率的な単離が可能になります。ここでは、肝再集団とTRAPのマウスモデルにおける流体力学的尾静脈注射を組み合わせた方法を示し、再入発性肝細胞の発現プロファイルを調べる。
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Cite this Article
Wang, A. W., Zahm, A. M., Wangensteen, K. J. Cell Type-specific Gene Expression Profiling in the Mouse Liver. J. Vis. Exp. (151), e60242, doi:10.3791/60242 (2019).
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