繊毛拍動頻度の定量化のための初代ヒト鼻上皮細胞モデルの収集、拡大、および分化
4.8K 回視聴
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9 件の引用
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11:13 分
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2021年11月10日
DOI :10.3791/63090-v
2021年11月10日
•4.8K 回視聴
Katelin M. Allan1,2 , Sharon L. Wong1,2 , Laura K. Fawcett1,2,3 , Alexander Capraro1,2 , Adam Jaffe1,2,3 , Cristan Herbert4 , Elvis Pandzic5 , Shafagh A. Waters1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales
このプロトコルでは、鼻上皮細胞の収集、拡張、器官型気道上皮細胞モデルへの分化、および生細胞イメージングとカスタムビルドスクリプトによる繊毛拍動頻度の定量化について説明します。
Chapters in this video
0:05
Introduction
1:16
Collection of Nasal Inferior Turbinate Brushings
2:25
Airway Epithelial Cell Expansion and Maintenance
3:23
Seeding and Differentiation of Airway Epithelial Cells and Maintenance of Differentiated ALI Models
4:12
Three-Dimensional Airway Epithelial Organoids
5:54
Imaging Cilia Beat Frequency
7:18
Data Analysis and Quantification of Cilia Beat Frequency
9:20
Results: Cilia Beat Frequency in Airway Epithelial Cell ALI Models of Cystic Fibrosis Patients and Healthy Participants
10:36
Conclusion
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