3D磁力アクチュエータと多機能蛍光イメージングを組み合わせて核メカノバイオロジーを研究

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5 件の引用

06:54 分

2022年7月5日

10.3791/64098-v

2022年7月5日

2.3K 回視聴

Miao Huang¹^,⁵ , Heyang Wang¹^,⁵ , Alfredo A. Delgado¹ , Tyler A. Reid¹ , Julian Long² , Shu Wang³^,⁵ , Hayley Sussman⁴ , Juan Guan⁵^,⁶^,⁷ , Hitomi Yamaguchi¹ , Xin Tang¹^,⁵^,⁸^,⁹

¹Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, ²Department of Materials Science and Engineering, University of Florida, ³Department of Biostatistics, University of Florida, ⁴Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), ⁵UF Health Cancer Center, University of Florida, ⁶Department of Physics, College of Liberal Arts and Sciences, University of Florida, ⁷Department of Anatomy and Cell Biology, College of Medicine, University of Florida, ⁸J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, ⁹Department of Physiology and Functional Genomics, University of Florida

本研究では、細胞質内に送達される磁気マイクロビーズを介して細胞核に直接力を加え、生細胞蛍光イメージングを同時に行うための新しいプロトコルを提示します。

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共焦点顕微鏡法

核メカノセンシング

磁性マイクロビーズ

細胞骨格力学

ライブセルイメージング

Chapters in this video

0:04

Introduction

0:36

Cell Culture with Magnetic Microbeads

1:29

Force Application and Live-Cell Imaging

4:34

Results: Application of Mechanical Force on Cell Nucleus and Simultaneous Live-Cell Fluorescent Imaging

6:28

Conclusion

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