3Dオルガノイドによる口腔食道扁平上皮癌のモデリング
4.3K 回視聴
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10 件の引用
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10:43 分
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2022年12月23日
DOI :10.3791/64676-v
2022年12月23日
•4.3K 回視聴
Samuel Flashner*1 , Cecilia Martin*1,2 , Norihiro Matsuura1 , Masataka Shimonosono1 , Yasuto Tomita1 , Masaki Morimoto1 , Ogoegbunam Okolo1 , Victoria X. Yu1,3 , Anuraag S. Parikh1,3 , Andres J. P. Klein-Szanto4 , Kelley Yan1,2 , Joel T. Gabre1,5 , Chao Lu1,6 , Fatemeh Momen-Heravi1,7 , Anil K. Rustgi1,5 , Hiroshi Nakagawa1,2,5
1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University
* These authors contributed equally
このプロトコルでは、化学的発がんによって誘発される正常、腫瘍前、および扁平上皮がんの病変を表すマウスの口腔食道3Dオルガノイドを生成および特性評価するための重要なステップについて説明します。
Chapters in this video
0:04
Introduction
1:28
Animal Dissection and Organ Collection
4:39
Isolation of Keratinocytes from the Dissected Mouse Tissue
6:07
Seeding of the Initial Cell Suspension
6:54
Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)
7:52
Preparation of Organoids for Paraffin Embedding
8:48
Results: Characterization of the Organoids
10:03
Conclusion
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