マウス卵巣エピゲノムとトランスクリプトームの細胞特異的ペアインプロゲーション

Overview

This study optimizes the translating ribosome affinity purification (TRAP) and isolation of nuclei tagged in specific cell types (INTACT) methods for paired interrogation of the ovarian transcriptome and epigenome in a mouse model. The approach enables analysis of specific ovarian cell populations without cell sorting, facilitating insights into cell type-specific changes associated with aging.

Key Study Components

Research Area

• Cell type-specific analysis
• Ovarian aging
• Transcriptomic and epigenomic investigation

Background

• Understanding ovarian aging is crucial for addressing female infertility.
• Current methods often require cell sorting, which can limit throughput.
• This technique offers a streamlined way to analyze ovaries from the same mouse.

Methods Used

• Optimized TRAP and INTACT methods
• NuTRAP mouse model crossed with Cyp17a1-Cre line
• Single ovary collection and specific protocols for nuclear isolation

Main Results

• Enabled paired analysis of transcriptomic and epigenomic data.
• Uncovered cell type-specific changes in the ovaries related to aging.
• Validated the potential of this method for broader application across various cell types.

Conclusions

• The method demonstrates effectiveness in isolating nucleic acids from specific ovarian cell populations.
• This approach has significant implications for studies focused on female reproductive health.

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