Name: Fusarium oxysporum f. sp. cubense Tropical Race 4 検出のための複合リコンビナーゼポリメラーゼ増幅 CRISPR/Cas12a アッセイ (動画) | JoVE Journal
Uploaded: 2025-11-14T13:35:00.000+00:00
Duration: 6 min 47 s
Description: 学ぼう、CRISPR/Cas12aとRPAを組み合わせたFusarium oxysporum f. sp. cubense Tropical Race 4の迅速検出法。携帯型蛍光計を用いた低コストで正確な植物病原体診断技術を習得できます。

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<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rapid+and+sensitive+detection+of+toxigenic+Fusarium+asiaticum+integrating+recombinase+polymerase+amplification%2C+CRISPR%2FCas12a%2C+and+lateral+flow+techniques%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">Rapid and sensitive detection of toxigenic Fusarium asiaticum integrating recombinase polymerase amplification, CRISPR/Cas12a, and lateral flow techniques.</a> Int J Mol Sci. 24 (18), 14134(2023).</li>
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</ol>

<table><tbody><tr><td>EnGen lba Cas12a(CFP1)タンパク質</td><td>ニューイングランド・バイオラボ</td><td>M0653T</td><td></td></tr><tr><td>EnGen lba Cas12a 希釈剤</td><td>ニューイングランド・バイオラボ</td><td>B0653A</td><td></td></tr><tr><td>エッペンドルフPCRチューブ</td><td>メルク</td><td>EP0030124332</td><td></td></tr><tr><td>エッペンドルフ・セーフロックマイクロ試験管</td><td>メルク</td><td>EP0030121708</td><td></td></tr><tr><td>エクストラクションバッグズ・ユニバーサル</td><td>ビオレバ</td><td>430100</td><td></td></tr><tr><td>GeneRuler 100 bp DNAラダー</td><td>サーモ・フィッシャー・サイエンティフィック</td><td>SM0241 / SM0242</td><td></td></tr><tr><td>ジーニー遠心分離機ミニ6KO</td><td>オプティジーン</td><td>オプフージ</td><td></td></tr><tr><td>ジーニーIII</td><td>オプティジーン</td><td>https://www.optigene.co.uk/instruments/genie-iii/&nbsp;</td><td></td></tr><tr><td>ジーニー・ストリップ・ホルダー</td><td>オプティジーン</td><td>GBLOCK-01 / GBLOCK-02 / GBLOCK-03</td><td></td></tr><tr><td>ジーニーストリップ</td><td>オプティジーン</td><td>OP-0008-50 / OP-0008-500</td><td></td></tr><tr><td>HiScribe T7 高収量RNA合成キット</td><td>ニューイングランド・バイオラボ</td><td>E2040S型</td><td></td></tr><tr><td>NanoDrop ND-1000 UV/VIS 分光光度計</td><td>サーモ・フィッシャー・サイエンティフィック</td><td>https://caeonline.com/buy/spectrometers/nanodrop-nd-1000/293669253</td><td></td></tr><tr><td>NEBuffer 3</td><td>ニューイングランド・バイオラボ</td><td>B7003S</td><td></td></tr><tr><td>NEBuffer r2.1</td><td>ニューイングランド・バイオラボ</td><td>B6002S</td><td></td></tr><tr><td>ポリビニルピロリドン(PVP)粉末</td><td>シグマ・オルドリッチ</td><td>9003-39-8</td><td></td></tr><tr><td>RNAクリーン&コンセントレーター-5キット</td><td>ザイモリサーチ</td><td>R1013</td><td></td></tr><tr><td>RNAローディングダイ</td><td>サーモ・フィッシャー・サイエンティフィック</td><td>R0641</td><td></td></tr><tr><td>スマートブルーブルーライトトランスイルミネーター</td><td>アキュリス・インスツルメンツ</td><td>E4000-E</td><td></td></tr><tr><td>水酸化ナトリウム(NaOH)ペレット</td><td>メルク、EMPARTA</td><td>K277</td><td></td></tr><tr><td>トリス(ヒドロキシルメチル)アミノメタン</td><td>メルク</td><td>77-86-1</td><td></td></tr><tr><td>TwistAmp リキッドベーシックキット</td><td>ツイストDx</td><td>TALQBAS01</td><td></td></tr></tbody></table>

fusarium oxysporum f. sp. cubense tropical race 4 検出のための複合リコンビナーゼポリメラーゼ増幅 crispr/cas12a アッセイ

Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4

Fusarium oxysporum f. sp. cubense Tropical Race 4 検出のための複合リコンビナーゼポリメラーゼ増幅 CRISPR/Cas12a アッセイ

Regular and accurate surveillance stands central to the efficient management of plant diseases. It can indicate which course of action is most ...

combined-recombinase-polymerase-amplification-crisprcas12a-assay-for

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DNAアセンブリを使用して、複数のCRISPRベクトルが単一クローニング反応に並列に構築することができ、CRISPR多数の構造を作ることは簡単な作業ベクトル。トマト毛状根は、CRISPRベクトルを検証し、突然変異体材料を生成するための優れたモデルシステムです。...

トマト毛状根の発生によるDNAの修飾のハイスループットCRISPRベクター構築と特性

我々は遺伝的にウスチラゴがmaydisスマット菌を操作する堅牢な遺伝子置換戦略を説明します。このプロトコルは、感染症の表現型を調べるために、欠失変異体を生成する方法について説明します。蛍光タンパク質タグをコードする配列を付加することによって、 例えば 、任意の所望の方法で遺伝子を修正するために拡張することができます。...

植物病原体の遺伝子操作<em&gt;ウスチラゴmaydis</em&gt;真菌生物と植物微生物相互作用を研究します

ここでは、CRISPR Cas9 ribonucleoproteins と相同性依存修理テンプレートを使用して線虫のゲノム エンジニア...

迅速かつ安易なパイプラインを生成する突然変異点ゲノムにc. の elegansを用いた CRISPR/Cas9 Ribonucleoproteins

このプロトコルの目的は、組み立てられたリボヌクレオタンパク質複合体とスクリーニングのためのdpy-10コクリスピーマーカーを使用して、C.エレガンスゲノムのシームレスなCRISPR /...

CRISPR/Cas9 dpy-10 Co-CRISPRスクリーニングマーカーと組み立てられたリボヌクレオプロテイン複合体を用いたC.エレガンスrbm-3.2遺伝子の編集。

本研究では、CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅に基づくカン ディダトゥス...

CRISPR-Cas12aと組み合わせたリコンビナーゼポリメラーゼ増幅を用いたフィールド展開可能な カンディダトゥス ・リベリバクター・アジアティカス検出(英語)

CRISPR-Cas系および抗CRISPRタンパク質は、 出芽酵母のブールゲートのスキームに統合されました。新しい小型論理回路は良好な性能を示し、dCas9/dCas12aベースの転写因子と抗CRISPRタンパク質の特性の両方の理解を深めました。...

CRISPR-Casシステムと抗CRISPRタンパク質に基づく遺伝子デジタル回路

DNAウイルスの微量検出のための組換えポリメラーゼ増幅とCRISPR/Cas12aシステムを組み合わせたプロトコルを提示し、ポイントオブケアDNAウイルス検出のための人工知能支援分類を備えたポータブルスマートフォン顕微鏡を構築します。...

RPA-CRISPR/Cas12A-SPMとディープラーニングに基づくDNAウイルス検出システム

ここでは、感染症病原体やその他の遺伝子マーカーの核酸バイオマーカーの検出に使用できる、プレミックス凍結乾燥リコンビナーゼベースの等温増幅反応とCRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)ベースの反応の段階的な調製について紹介します。...

プレミックス試薬とフリーズドライ試薬によるポイントオブケアCRISPRベースの診断

アシネトバクター・バウマニーの迅速かつ正確な検出を容易にするために、A.

Recombinase Polymerase Amplification/Cas12aベースシステムを用いた Acinetobacter baumannii 感染症の迅速かつ特異的な検出

ここでは、増幅可能な細胞溶解、リコンビナーゼポリメラーゼ増幅、およびCRISPR/Cas12aアッセイを使用して、 Lotmaria passim や Nosema ceranae...

RPA/CRISPR/Cas12aシステムへのアルカリ溶解によるトリパノソーマ科寄生虫および Nosema ceranae のオンサイトDNA検出

学ぼう、CRISPR/Cas12aとRPAを組み合わせたFusarium oxysporum f. sp. cubense Tropical Race 4の迅速検出法。携帯型蛍光計を用いた低コストで正確な植物病原体診断技術を習得できます。

Fusarium oxysporum f. sp. cubense Tropical Race 4 検出のための複合リコンビナーゼポリメラーゼ増幅 CRISPR/Cas12a アッセイ

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