JOVE JOURNAL

Biochemistry

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Quantitative Analysis of Dietary Vitamin A Metabolites in Murine Ocular and Non-Ocular Tissues Using High-Performance Liquid Chromatography

Matthias Leung¹, Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Drew Yochim¹, Swati More², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²Center for Drug Design,University of Minnesota

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Journal (Biochemistry)

NMR ¹⁵N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins

Tobias Stief^1,2, Katharina Vormann^1,2, Nils-Alexander Lakomek^1,2

¹Institute of Biological Information Processing (IBI-7),Forschungszentrum Jülich, ²Institute of Physical Biology, Faculty of Mathematics and Natural Sciences,Heinrich Heine University Düsseldorf

Journal (Biochemistry)

Evaluation of the Effect of Growth Factors on Chlorophylls a and b Production from Microalgae

Frizek Nathaniel Morales-Rivera¹, Sofia Torres-Momber¹, Julio César Jacuinde-Ruíz¹, Juan Cristóbal Camacho-Arriaga¹, Juan Carlos González-Hernández¹

¹Chemistry and Biochemistry Engineering Department, Technological Institute of Morelia,National Institute of Technology of Mexico

Journal (Biochemistry)

Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells

Connie Chen*¹, Katherine A. Rafael*¹, Ginam Cho¹, Youngshin Lim^1,2

¹Department of Pathology,Cedars-Sinai Medical Center, ²Department of Biomedical Science Education, College of Medicine,Charles R. Drew University of Medicine and Science

Journal (Biochemistry)

Optimizing Tubulin Yield from Porcine Brain Tissue

Adam Norek¹, Michaela Šťastná¹, Jan Gečnuk¹, Pavel Kulich¹, Jan Gebauer¹, Lubomír Janda¹

¹Veterinary Research Institute

Journal (Biochemistry)

Methodology for Studying Interactions of Vitamin A Membrane Receptors and Opsin Protein with their Ligands in Generating the Retinylidene Protein

Rakesh Radhakrishnan¹, Anjelynt Lor¹, Dorothy Li¹, Deepti Mudaliar², Glenn P. Lobo¹

¹Department of Ophthalmology and Visual Neurosciences,University of Minnesota, ²High Throughput Screening Laboratory, Institute for Therapeutics Discovery & Development,University of Minnesota

Journal (Biochemistry)

Demonstration of Membrane Transport of Histidine using Goat Intestinal Inverted Sacs: An Experiential Pedagogical Tool for Undergraduates

Hiba Haris¹, Prachi Kumar¹, Vivek Bhardwaj¹, Sidhartha Taritla¹, Vandana Malhotra¹, Nandita Narayanasamy¹

¹Department of Biochemistry, Sri Venkateswara College,University of Delhi

Journal (Biochemistry)

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Cameron McClymont¹, Karolina Chabowska¹, Sherry Xie¹, May Thu Kyaw¹, Hasan Yardimci¹

¹The Francis Crick Institute

Journal (Biochemistry)

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels

Yue Zhao¹, Mai Hu², Yiwen Gong², Yihao Zhao², Zhiwei Shen²

¹The First Affiliated Hospital of Nanchang University, ²The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine,Nanchang University

Journal (Biochemistry)

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Htet Ng*¹, Masuda Begum*¹, Gabriella N. L. Chua*^1,2, Shixin Liu¹

¹Laboratory of Nanoscale Biophysics and Biochemistry,The Rockefeller University, ²Tri-Institutional PhD Program in Chemical Biology

Journal (Biochemistry)

Analyzing Telomeric Protein-DNA Interactions Using Single-Molecule Magnetic Tweezers

Han Gao¹, Yanling Liu¹, Zhongbo Yu¹

¹State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy,Nankai University

Journal (Biochemistry)

MultiBac System-Based Purification and Biophysical Characterization of Human Myosin-7a

Marvin Wright*¹, Shayna Redford*¹, Jacob Vehar¹, Kevin C. Courtney¹, Neil Billington^1,2, Rong Liu¹

¹Department of Biochemistry & Molecular Medicine, School of Medicine,West Virginia University, ²Microscope Imaging Facility,West Virginia University

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