Intranasal Infection of a Mouse with a Pathogenic Influenza Virus

초록

Source: Wang, M., et al, Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice. J. Vis. Exp. (2020).

This video demonstrates intranasal infection of a mouse with a pathogenic influenza virus to establish a respiratory tract infection. The procedure outlines the administration of the virus into the nasal cavity. The virus enters host cells, replicates, and activates the immune system.

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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

NOTE: Virus infection of mice and isolation of organs should be performed under Animal Biosafety Level 2 (ABSL2) conditions.

1. Inoculation of PR8 influenza virus and recording of mice weight

1. Prepare 8-week-old male C57BL/6 mice for infection in the ABSL2 room.
   NOTE: This protocol is also suitable for experiments with female mice.
2. Dilution of PR8 virus: take out the virus from the -80 °C freezer and incubate on ice until it melts into liqu....