이차 부착 방법에 의한 쥐 뼈로부터 단핵구-대식세포 계통 세포의 분리
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Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| 35 mm<sup>2</sup> cell climbing slices | NEST Biotechnology | 80102 | |
| Anti-cathepsin K | Abcam | ab19027 | 1:1,000 |
| Anti-CD11 isotype control | Abcam | ab172730 | 1 μg/test,1.675 mg/Ml |
| Anti-CD11b/c | Absin | abs124232 | 1μg/test, 1 mg/mL |
| Anti-TRAP | Abcam | ab191406 | 1:1,000 |
| Anti-β-actin | Beyotime | AF5003 | 1:1,000 |
| Cell climbing slices | NEST Biotechnology | 80102 | |
| Cell culture dish | corning | 430167 | |
| Cell culture flask | corning | 430168 | |
| Dulbecco's modified eagle medium (DMEM) | Gibco | C11995500BT | |
| Fetal bovine serum (FBS) | Gibco | 10099141C | |
| Goat anti-rabbit IgG | Abcam | ab150077 | for IF, 1:2,000 |
| goat anti-rabbit IgG | Abcam | ab6721 | for WB, 1:2,000 |
| M-CSF | Pepro tech | 400-28 | |
| PBS | Biosharp | BL302A | |
| RANKL | Pepro tech | 400-30 | |
| SD rat | Shanghai SLAC Laboratory Animal Co, Ltd | 1-10 days old | |
| SDS-PAGE gel preparation kit | Solarbio | P1200 | |
| TRAP/ALP Staining Kit | Wako | 294-67001 | |
| Trypsin-EDTA solution | Biosharp | BL512A | |
| Wright-Giemsa solution | Keygen Biotech | KGA225-1 |
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Cite This Article
Jin, X., Li, Y., Chen, X., Chen, J., Xu, J. Isolation of Monocyte-Macrophage Lineage Cells from Rat Bones by Secondary Adherence Method. J. Vis. Exp. (185), e64053, doi:10.3791/64053 (2022).